Study On Expression Of Fas/FasL System In Placenta From Pregnancy Complicated With Pregnancy Induced Hypertention

The Fas molecule ( designated as CD95 or APO-1) is a cell surface receptor belonging to the nerve growth factor receptor-tumor necrosis factor recepter family of apoptosis -signaling molecules, that can induce apoptotic cell death in sensitive cells. The triggering of Fas can be upon binding by ite natual ligand of Fas. Activated lymphocytes are removed by a Fas/FasL-mediated programmed cell death process called activation induced cell death (AICD), which is the very important to fatal-maternal immune intolerance and embryo or placenta's development. Abnormal expresion of Fas/FasL in the uterus-placenta tissue must be induced the fetal-materal immunologic disorder that redulted in abnormal immunoregulation ,injured of trophoblast and paternal development . While, it correlates well with spontaneous abortion, intrauterine growth restriction, pregnancy induced hypertention (PIH) and so on. May be it is one of the important mechanism of the etiology of PIH .Objective: To study the expression of Fas/ FasL in placenta from pregnancy complicated with PIH, and analyze the immunological mechanism of PIH further is the experiment's purpose.Method: Use the irnrnunohistochemistry to determine the location and intensity of Fas/ FasL staining in placenta from 20 cases of normal pregnancy and 65 cases of mild , moderate and severe group of pregnancies complicated with PIH. The clinical date is analyed by T test and the ordinal date is analyed by two Independent Sample Test.Result:1.Immunolocalization of Fas/ FasL in all cases was observed mainly in syncytiotrophoblast and decidual cells.2. Expression of Fas in syncytiotrophoblast and decidual cells: Indensty of Fas immnostaining in syncytiotrophoblast and decidual cells was signifantly elevated in PIH group (Ps=0. 001, Pd=0. 004) compared with the normal group . The Fas positiveexpression of every group in syncytiotrephoblast was different (Hc=20. 051, P<0. 01). There was different between the moderate, severe Pffi group ( Pm<0. 05; Ps<0. 01)and the normal group , but there was no qualitative differences between the mild PIH group (P>0. 05) and the normal group. The Fas positive expression of every group in decidual cells was different (Hc=17. 647, P=0. 0001) . There was different between the severe PIH group(P<0. 01) and the normal group , but there was no qualitative differences between the mild, mederate PIH group and the normal group(P>0. 05).3.Expression of FasL in syncytiotrophoblast and decidual cells: Indensty of FasL immnostaining in syncytiotrophoblast and decidual cells was signifantly reduced in PIH group(Ps=0. 000, Pd=0. 015) compared with the normal group. The FasL positive expression of every group in syncytiotrephoblast was different ( Hc=22.343 , P=0. 000) . There was different between the moderate , severe PIH group (Pm<0. 05; Ps<0. 01)and the normal group ,but there was no qualitative difference between the mild PIH group (P>0. 05)and the normal group. The FasL positive expression of every group in decidual cells was different (Hc=15. 163, P=0. 002). There was different between the the severe PIH group (P<0. 05)and the normal group, but there was no qualitative differences between the mild, mederate PIH group and the normal group (P>0. 05) .Conclusion: The elevated expression of Fas and the reduced expression of FasL on placenta trophoblasts of PIH groups which destroy the immunologic tolerance between the mother and the fetus redult in the damage of immuno-pathological of placenta , and consequently lead to an increased apoptosis in trophblasts. Not only induced immuno-pathology impair and abnormal palcental development but also intolerence of fetal-matermal immune system . At last, it redulted in PIH and other ills.