| Objective: Effective activation and differentiation of anti-tumor T cells required at least a second antigen-independent costimulatory signal provided by different accessory molecules on the surface of APC. Multiple observations suggest that DCs in tumors are functionally impaired, which might be due to production of immunosuppressive factors by the tumor. Such as DCs are in an immature stage or minimally activated and fail to express CD80/86.In our study, we using flow cytometry to detect the expression of CD83, CD80, CD86 in the surface of dendritic cells cultured from the peripheral blood from patients compared with health donors.Methods: 5 Patients with EC and 5 age-matched healthy individuals were enrolled in this study. DC was generated from PBMNC according to the methods described by Romani et al. After 7 days, thecultures were collected, we using electronic microscopy to observe the cells, and using flow cytometry to detect the expression of CD83, CD80, and CD86 in the surface of dendritic cells, the ability of DC to induce T lymphocyte proliferation were evaluated by a liquid scintillation counter. Results: 1. Compared with healthy donors, dendirtic cells from theperipheral blood of patients expressed the lower CD80 and CD86. And the ability of DC in patients to induce T lymphocyte proliferation was singnificantly lower than that of controls. 2. There's no difference in the expression of CD83 between patients and donors.Conclusion: The immunology function of dendritic cells cultured from peripheral blood of the esophageal patients was impaired during the pathogenesis.
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