The Mucosal Protective Effect On Gastric Mucosa Inflammatory Disease

Gastric mucosa is exposed to the HC1, pepsin, bile acids, steapsin, ethanol and NSAISs. But in normal conditions, gastric mucosa can resist above aggressive factors, because which has a series of defense mechanisms such as unstirred layers of mucus and bicarbonate, continuous layers of surface epithelial cells (secreting mucus, bicarbonate, and prostaglandins), cell renewal, mucosal microcirculation, ongoing generation of prostaglandins and growth factors. Ulcerations develop as a result of an imbalance between aggressive factors present in the gastric lumen and mucosal defense. Gastric mucosal protection play a crucial role in the gastric mucosa Inflammatory disease development, such as gastric ulcer and chronic gastritis. Recently, gastric mucosal protective agents have been paid much attention to. This experiment chosed 2 kinds of gastric mucosal protective agents: Teprenone and muscovite and prepared 2 kinds of models: Acetic acid-induced ulcers and chronic ulcers to explore the gastric mucosal protective effect on gastric mucosa Inflammatory disease.Materials & Methods120 healthy, sexual-mature, and male wistar rats were adpopted to our research.Model 1 (70) : Acetic acid-induced ulcers were prepared as follows: The animals were deprived of food but allowed free access to drinking water for 24h prior to experiment. Under pentobarbital anesthesia, a midline epigastric laparotomy was made. After exteriorizing the stomach, acetic acid solution (0.05 ml) was injected into the submucosal layer at the region between the fundus and the pylorus on the anterior wall. Then the abdomen was closed. The concentrations of acetic acid solution used were 20%. The rats were divided into 6 groups as follows at 3d after ulcer induction. Model group: the rats was killed then; Control group: the rats received intragastrically (gavage) normal saline 2 ml twice daily for 14 days; Teprenone group: the rats received intragstrically (gavage) teprenone 200mg/kg d in the morning and normal saline 2 ml in the afternoon daily for 14 days; Omeprazole group: the rats received intragstrically (gavage) omeprazole 15mg/kg d in the morning and normal saline 2 ml in the afternoon daily for 14 days; Teprenone + omeprazole group: the rats receivedintragstrically (gavage) teprenone 200mg/kgd in the morning and omeprazole 15mg/kg d in the afternoon daily for 14 days; Muscovite group: the rats received intragastrically (gavage) muscovite 160mg/kgd twice daily for 14 days. We observed the common manifestion and mucosal morphology of the rats, measured and calculated the ulcer index and cumulative healing rate. After that, the blood samples were obtained to measure the PGE2 and EGF levels, the gastric tissue samples were obtained to measure the hexosamine level. Perpendicularly oriented paraffin sections were stained with HE, VG, AB-PAS. The immunohistochemical assessments of expression of EGFR and bFGF were made by the image analysis.Model 2 (50) : Chronic gastritis were prepared as follows: The rats received intragstrically (gavage) 2% sodium salicylate 2 ml daily in previous 3 weeksl4 days; The rats were allowed free access to food or water, but were prived of food but allowed free access to drinking water on odd days and no access to drinking water on even days in another 3 weeks; In first week, the rats drank 20 mmol/L sodium deoxycholate in stead of water. After induction of chronic gastritis, the rats were divided into 4 groups as follows: Model group: the rats received intragstrically (gavage) normal saline omeprazole daily for 2 weeks; ''999" herbs group: the rats received intragstrically (gavage) "999" herbs 167mg/kgd daily for 2 weeks; Low dose muscovite group: the rats received intragstrically (gavage) muscovite 60mg/kg d daily for 2 weeks; middle dose muscovite group: the rats received intragstrically (gavage) muscovite 120mg/kg d daily for 2 weeks; High dose muscovite group: the rats received intragstrically (gavage) muscovite 240mg/kg d daily for 2 weeks. We also set up a normal group: the rats were well fed for...