| Objective The Experimental Allergic Encephalomye litis(EAE) is the most suitable animal model for investigation in the pathogenesis and the treatment of EAE. The expressions of ICAM-1 ,VCAM-1 and nestin in Wistar rat brain of acute EAE were investigated to study the purpose and significance of their expressions in EAE rat brain .Methods All experimental Wistar rats were inoculated intradermicly in feet with emulsion contained guinea pig spinal cord homogenate and complete Freund's adjuvant.At first,we observed the clinical manifestation and the pathological changes with the aid of light and electron microscope to check the success of the model. Then,the expressions of ICAM-1 ,VCAM-1 and nestin were detected by the method of immunohistochemisty.The results of their expressions were compared by T test with control .Result EAE model showed typical clinical situation, pathological change and ultrastructure of EAE.Significant difference existed between the experimental group and controul in the expressions of ICAM-1 ,VCAM-1 and nestin(P＜0.01).Conclusion The method of establishing the EAE model is stable and reliable.ICAM-1 and VCAM-1 may play a key role on the onset of EAE.The significant reinforcement of Nestin expression indicates that hyperplasia of neural stem cell in EAE rat brain ,and it may offer some substances for the repair of CNS. |
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