Microsatellite Change In Bladder Transitional Cell Carcinoma And Its Mechanisms

Objective To study the expression of microsatellite change in bladder transitional cell carcinoma(BTCC) and its relationship with gene mutation. Methods Tumor samples from 40 patients with BTCC were analyzed for microsatellite change by PCR . The same way was used to check the mutations of BAX gene and TGF- βR II gene in BTCC. Results In 33 of 40 BTCC patients (82.5%) loss of heterozygosity(LOH) or microsatellite instability(MSI) were found on at least one microsatellite locus. The positive rate of D9S162, D16S476, D9S54, FGA and IFN-A1 was 57.5%(23/40) , 42.5%(17/40) , 37.5% (15/40) , 47.5%(19/40) and 55%(22/40) respectively, which was higher than of the benign diseases. However, it had no relationship with stage and grade of BTCC. Frame shift mutation of BAX gene and TGF- β RII gene was found in 14 and 11 of the 33 cases with microsatellite change respectively, but it was not found in microsatellite change-negtive BTCC and control tissue. Conclusion It is a efficient way to diagnose BTCC early and supervise its recurrence through detecting the microsatellite changes. The effect of gene mutation on tumorgeneration may be related to microsatellite changes.