Expression And Significance Of HSP70 In Larynegeal Papilloma

It is found that HsP 70 ( Heat shock protein 70 ) has a relationship with the development of neoplasm and plays an important role in the neoplasm - immune. HsP is a kind of molecular companions . It can help the protein construct and stablize in the course of hyperplasia to adapt the bad enviroments . The expression of HsP in malignant tumor is higher than that in the others. But there is not any report about it in larynegeal papilloma (LP) .We chose thirty cases of LP, used immunohistochemistry method to test the expression of HsP 70 in it and analyses the clinical value.Materials and MethodsMaterials; Choose thirty cases of LP in CMU 2 from 1989 to 2002. Among these, male 16 cases, female 14 cases. Age from 20 to 72 years old, average 46 years old. At the same time , choose 30 cases of vocal polyps and 11 cases of vicious larynegeal papilloma as control.Main reagents and instruments; The antibody we used is HsP70 single - leaf antibody (mouse antihuman) ; And the SP - box is a chemical reagent box which came from FU JIAN MaiXin company. The other reagents and instruments inclde PBS buffer; 10% normal goat serum;DAB colour reagents;the different concentration ethyl from70 - 100% ; fridge; lukewarm box and photograph collect system.Methods; Use immunohistochemistry method to examine the expression of HsP 70 in larynegeal papilloma. Conventional getrid of paraffin wax and water. After immersed in 3% H202 and normal goat serum,the sections were incubated with antibody to HsP70 at 37℃ overnight. A biotinylated anti -rabbit antibody was used as the second antibody. For negative controls the primary antibody was omitted on one of the mounted sections on all slides. Processing was ultimatelyperformed with a SP and DAB. The reaction was observed under a light - microscope and was finally stopped by application of PBS. The specimens were dehydrated in baths of ascending ethanol concentration. Analysis and photograph of the sections under Olympus light - microscope . Thex2 - test was used to a-nalysis the results in statistics.ResultsNine cases are positive in 30 cases of LP (30% ) ; one case in 30 cases of vocal polyps (3. 33% ) is positive . There is a difference between them in statistics. Eight cases are positive in 11 cases of vicious larynegeal papilloma (72. 7% ). There are differences between vicious larynegeal papilloma and LP (p < 0.05).DiscussionHsP is a kind of proteins which exists in many cells. After stimulated by alcohol and virus infection and so on , it can be induced to express. HsP 70 has a closely relationship with the development of tumor cell. We found from tests that in larynegeal papilloma and vicious larynegeal papilloma, the expression of HsP 70 is higher clearly, which may have to do with the lack of nutrition of tumor cells and have an association with the HsP 70 as a molecular companion to stablize the abnormal hyperplasia in rapid grow of tumor cells. HsP can make the epithelial cells grow continuously and turn to molignant ones.ConclusionsHsP 70 may have an important role during the development of larynegeal papilloma.