The Research Of The Therapy Effect Of The Cerebral Infarction And The Influence Of VEGF By Injecting PLXSN-bcl-2 Into The Rats' Lateral Ventricle

Objective1 To observe the therapy effect of the cerebral infarction and the influence of the expression of VEGF by injecting the PLXSN - bcl - 2 plasmid recombinant into the rats'lateral ventricle.MethodFirstly, we put 180 Wistar rats which have been made into MCAO 2h models by zea - longa suture embolization into the groups of saline, vacant PLXSN plasmid and PLXSN - bcl - 2 plasmid. Secondly, we inject saline, vacant PIX-SN plasmid and PLXSN - bcl -2 plasmid into the rats'lateral ventricle soon after infarction. Finally, we detect the cerebral infarction volume, the expression of bcl - 2 and VEGF protein after 3, 6, 24, 48, 72 hours of cerebral ischemic reperfusion by TTC staining, image analysis system, digital camera and the method of immunohistochemistry. All the data are described by x s and examined by one - way analysis of variance and T test.ResultsThe infarction volumes of different reperfusion time point which were detected by image analysis system and TTC staining. The infarction volume of the bcl - 2 group is much smaller than the other two groups at the same time point ( P < 0.01) ,and the volume of the three groups increased gradually with the reperfusion time and can not achieve the peak value in 72 hours. The therapy effects between the saline group and the vacant plasmid group have no difference (P >0.05 ) , and the effect of the bcl - 2 group has obvious difference to the other two groups mentioned above( P <0.01).The cell percentage of the positive expression of bcl - 2 protein detected by the microimage analysis system and immunohistochemistry. The expressed bcl -2 protein of the bcl - 2 group is far more than the other two groups at the same time point ( P < 0.001). The quantity of expressed bcl - 2 protein of the saline and the PLXSN plasmid groups increased gradually at the first 3 hours, and it a-chieved the peak value at the 48th hour, and then it decreased gradually. The quantity of the bcl - 2 group increased slowly in the first 6 hours, and it enhanced quickly after 6 hours, achieving its peak value at the 24 hour, and then it decreased little by little. The expression of bcl - 2 protein between the saline group and the vacant plasmid group have no difference (P >0.05) , and the expression of bcl - 2 protein of the bcl - 2 group has obvious difference to the other two groups mentioned above ( P <0.01) .The cell percentage of the positive expression of VEGF protein detected by the microimage analysis system and immunohistochemistry. The expressed VEGF protein of the bcl - 2 group is far more than the other two groups at the same time point ( P < 0. 001). The quantity of expressed VEGF protein of the saline and the PLXSN plasmid groups increased gradually at the first 3 hours, and it achieved the peak value at the 24 hour, and then it decreased gradually. The quantity of the bcl -2 group increased quickly from the 3th hour, achieving its peak value at the 24* hour, and then it decreased little by little. The expression of VEGF protein between the saline group and the vacant plasmid group has no difference ( P > 0. 05 ) , and the expression of VEGF protein of the bcl - 2 group has obvious difference to the other two groups mentioned above ( P < 0. 01).ConclusionThe method of injecting PLXSN - bcl - 2 into the rats' lateral ventricles has obvious therapy effect to ischemic cerebral infarction, and it promote the expression of VEGF protein greatly. The bcl - 2 protein was expressed effectively.It can take effect at earlier period and can last for a long time.