Study On The Expression Of Nm23-H1 Gene And CDNA Gene Mutation Of Nm23-H1 Gene In Human Hepatocellular Carcinoma

Objective To detect the expression of nm23-H1 and its cDNA gene mutation in hepatocellular carcinoma (HCC), and study the role of nm23-H1 in the pathogenesis of HCC and elucidate the molecular biology mechanism of hepatocellular carcinoma metastasis. Methods Using semi-quantitive reverse transcriptase-polymerase chain reaction (RT-PCR) and single strand conformation polymorphism (SSCP) , the expression variation and cDNA gene mutation of nm23-Hl gene were detected in 30 HCC tissues, their corresponding adjacent liver tissues and in normal liver tissues of 16 non-liver disease patients. Results 漏the expression rate of nm23-Hl RNA was 100% in the HCC tissues , their corresponding adjacent tissues and in the normal liver tissues. There were no significant difference in the expression rate of nm23-H1 gene among the three kind of tissues(P>0. 05); (2)The expression level of nm23-Hl gene in the HCC tissues, their adjacent tissues and normal liver tissues was 1.619 0.407( x s), 1.302 0.343( x s) and 1.232 0.386( x s), respectively. The expression level of nm23-H1 gene in the HCC tissues was singnificantly lower than that in the adjacent and normal liver tissues (P <0.01); (3) The expression level of nm23-H1 gene in the low-differentiated HCC was much lower than that in the high and middle differentiated HCC tissues (P< 0.01); (4) The expression level of nm23-Hl gene in HCC tissues with satellite focuses was not significantly different from that without satellite focuses (P > 0. 05) ; (5) The expression quantity of nm23-H1 gene in HCC tissues was not closely related to HCC patients' age and gender (P>0. 05) ; (6) None of the nm23-Hl gene mutation was found in all the hepatocellular carcinoma samples. Conclusion (1)The negative expression rate of nm23-H1 in the HCC tissues is very low; (2) The expression level of nm23-H1 in the HCC tissues is much lower than that in the adjacent and normal liver tissues, which indicates that the inactivation of nm23-H1 is associated with thepathogenesis of HCC; (3)The expression level of nm23-Hl gene is highly associated with the pathological differentiation of HCC, which indicates that the alternation of nm23-Hl can serve as a prognostic indicators in the progression of HCC; (4)The expression level of nm23-Hl gene in HCC tissues was not closely related to HCC patients' age, gender and whether there are satellite focuses in HCC tissues; (5) The cDNA gene mutation rate of nm23-Hl in the HCC tissues is very low.