Experimental Study On Protective Action And Mechanism Of Scallp Skirt-glycosaminoglycan On Vascular Endothelial Cell Damage

Objective To investigation the protective action of the extract of scallop skirt-glycosaminoglycan(SS-GAG) on the endothelial cell , and to discuss it's mechanism of anti-atherosclerosis(AS).Methods 1. The endothelial cell of human umbilical vein had been cultured in vitro, and induced by oxygen-derived free radidicals or polycatholyte , then the damage model had been established. MTT chromatometry was used to study the anti-effect of SS-GAG on the endothelial cell damage and proliferation activity.2. Oxidative damage to vascular endothelial cell was caused by Fenton reaction, to observe the effect of SS-GAG on the lactate dehydrogenase(LDH) secret of the endothelial cell.3. To observe the action of SS-GAG on the secret of endothelins and angiotensin II in the Fenton architecture by the means of radio-immunity.4. The effect on the expression of vascular cell adhesiveness molecul-1(VCAM-1) and platelet derivation growth factor(PDGF-BB) had been observed by the means of ELISA after the endothelial cell oxidizing damage had been caused by Fenton architecture.Results 1.In positive groups, polylysine, damaged by Fenton reaction , the endothelial cell proliferation dereased remarkably (P<0.01) . While pretreated by SS-GAG, cell proliferation damages lowered obviously (P < 0.05 ) , and cell proliferation in large dose SS-GAG protective control groups was higher than that in less dose control groups (P<0.05) .2. LDH excretion of the endothelial in HUVEC cellculture medium increased remarkably when the endothelial cell damaged by oxygen-derived free radidicals induced by Fenton reaction, but it decreased remarkably when pretreated by SS-GAG(P<0.01) .3. Compare with positive control group damaged by oxygen-derived free radidicals , the excretion of endothelins decreased remarkably in SS-GAG group (P<0.01) , and decreased with increase of SS-GAG dose. After damaged by oxygen-derived free radidicals, the excretion of angiotensin II in HUVEC rised obviously (P<0.01) .Anpotensin II excretion in large dose SS-GAG protective control groups was less than that in small dose control groups (P<0.01) .4. Compare with positive control group, the number of VCAM-1 expression descended obviously in SS-GAG groups (P<0.01) .5. Compare with positive control group, the number of PDGF-BB expression descended obviously in SS-GAG groups (P<0.05) .Conclusion SS-GAG can reduce the endothelial cell damage caused by oxygen-derived free radidicals or polycatholyte, can restrain the excretion of endothelins and angiotensin II, and reduce the expression of VCAM-1 and PDGF-BB. These indicate SS-GAG has satisfactory protection effect on the endothelial cell damage, and suggest the antiatherosclerosis mechanism of SS-GAG may has relation to its protection on the endothelial cell, its inhibition of endothelins and angiotensin II excretion, and reduction of VCAM-1 and PDGF-BB expression.