| Ovarian cancer is the commonest malignancy of the female genital tract.The early stages of malignant growth in the ovary do not usually produce symptoms and the biologic rnornitoring markers usually lack ,so late diagnosis and ineffective treatments are probably the main reasons for the poor prognosis.Ovarian cancer has a relationship with hereditary agent, environmental agent, endocrine agent,but the specific mechanism of carcinogenesis is a multistage,progressive process such as activation of oncogenes and inactivation of anti-oncogenes.In spite of significant advances in surgery and the use of new,more effective chemotherapeutic regimens,that late stage ovarian cancer is hard to cure forces us to explore new ways. With the lucubration of the carcinogenesis, it was gradually realized that angiogenesis had a close correlation to carcinoma. The growth and progression of tumors is dependent on the process of angiogenesis,which provides oxygen and nutrient and cleans metabolite for the tumors.In the cells of primary maligmant tumors,a lot of positive and negative regulators evoke and regulate the formation of new blood vessel ,and tumor microvessles net.The "angiogenic switch" is very necessary for the growth rate of tumors. Vascular endothelial growth factor (VEGF) can enhance the permeability of venule and venous.lt can also accelerate the division and proliferation of endothelial cells,which will make calcium aggregation in cytoplasm ,induce new blood vessel formation and growth, invasion and metastasis of tumors. Endostatin was first isolated from the supernatant of an in vitro culture of a murine hemangioendothelioma cell line (EOMA cells) by O' Reilly et al. Endostatin can inhibit the growth of endothelial cells in vitro,and systemic administration has beenshown to inhibit the growth of established tumors and metastasis in vivo. In this studay,the expression of endostatin and VEGF have been examined,and their cliniclpathological correlations have been analysed in a series of 63 epithelial ovrian cancers and 19 normal ovarian samples.Materials and Methods35 patients with primary epithelial ovrian cancers who were diagnosed and treated at Gynecological department, The First Affiliated Hospital of Zhengzhou University ,China.between 2001-2003 were entered into the current study.Among them 7 were stage I ,5 were stageII, 15 were stage 111,8 were stage IV. Histologically, 22 were serous ovarian cancers,8 were mucinous ovarian cancers,5 were endometrioid ovarian cancers,9 were grade I ,18 were grade II ,8 were gradeIII.13 normal ovrian samples were collected from patients operated for uterine leiomyoma and adenomyoma.These samples were immediately frozen and stored at -80 癈.In addition to the above materials, paraffin blocks from 28 epithelial ovarian carcinomas and 6 normal ovarian samples were retrieved from the files of The Department of Pathology, The First Affiliated Hospital of Zhengzhou University, China. All the tumour patients did not receive radiotherapy or chemotherapy before operation. 82 specimens were fixed in buffered-formalin and embedded in paraffin for routine pathological examination and immunohistochemistry.AH the samples hadn't received radiotherapy or chemotherapy.RT-PCR and immunohistochemistry staining were used to detect the expression of endostatin and VEGF in normal ovarian and epithelial ovarian cancer samples. Results1. The levels of endostatin and VEGF mRNA expression in epithelial ovarian cancers were higher than those in normal ovaries (P<0.05); The ratio of endostatin and VEGF was higher in the normal ovaries (>1)than in ovarian cancers(0.05);4. The level of endostatin mRNA expression in moderately differentiated ovariancancers was higher than that in well and poorly differentiated ov...
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