Effect On Chorial And Decidual Progesterone And Estrogen Receptor And Apoptosis Control Gene By Mifepristone In Artificial Early Pregnancy

Objective: The theorem of Mifepristone as an antiprogestin is widespread confirmation, but the concrete action manier of Mifepristone to progesterone and estrogen receptor is no agreement among many views. Recently, some scholars suggest the new standpoint that Mifepristone could accelerat apoptosis of decidual and chorial cell. The study assay progesterone and estrogen receptor and apoptosis control gene in Mifepristone group and contrast group, to clear-cut the mechanism that Mifepristone evoke abortion pass apoptosis control gene and concrete action manier of Mifepristone on progesterone and estrogen receptor.Methods: Contrast group 15 chorial and decidual superscript from women that are performed artificial abortion voluntary; Mifepristone group 15 chorial and decidual superscript from women that are performed artificial abortion after taking Mifepristone two days voluntary ,their cyesis period is all 50 to 60 days.To assay the protein expression of Bcl-2,Bax,Fas,FasL in chorial and decidual tissueby immunohistochemistry;to assay the level of PR and ER by immunohistochemistry and radioligand binding assay (RBA).Results: Fas protein main distribut on cytotrophoblast and bit distribut on syncytiotrophoblast and decidual cell, there is not significant discrepancy in Mifepristone group and contrast group(P>0.05). FasL protein main distribut on syncytiotrophoblast, expression of FasL was obviously weaker in Mifepristone group than contrast group(P<0.05);Bcl-2 protein main distribut on cytotrophoblast and decidual cell, expression of Bcl-2 has not obviously difference in Mifepristone group and contrast group(P>0.05);Bax protein distribut on cytotrophoblast,syncytiotrophoblast and decidual cell, expression of Bax was obviously stronger in Mifepristone group(P<0.01);expression of PR was obviously stronger in Mifepristone group than contrast group by immunohistochemistry (P<0.01),expression of ER was also stronger in Mifepristone group than contrast group by immunohistochemistry(P<0.05);the level of PR is lower(P<0.01) and ER is higher(P<0.05)in Mifepristone group than contrast group by RBA. Conclusion: 1.Mifepristone evoke abortion pass FasL-T lymphocytic cell immuinity pathway: expression of FasL on syncytiotrophoblast cell decrease and could not bind with Fas on T lymphocytic cell to induce activat T lymphocytic cell increasing in mother proper and mother proper's reject reaction to embryo. That is Mifepristone evoke abortion may be include immuinity agent.2.Mifepristone evoke abortion pass dysequilibrium of Bcl-2/Bax apoptosis control pathway: the increasing of Bax induce dysequilibrium of Bcl-2/Bax and Accumulation of Bax/Bax ,at last it evoke cell apoptosis .3.Mifepristone resist progesterone to make cell that contains ER has unresist estrogen effect and increase expression of ER,PR, Mifepristone bind with PR competitively and oppose progesterone's action in early pregnancy.