| IntroductionBecoming a tumour is a very complepxity process, many genes take part in. following the modem theory and technology of cell - molecular biology developed, human have new knowledge about the regular of cell turn to cancer or tumour. Believe that the cycling of cell is basic process in living life. In cycling cell become multiply , polarization , senescence or death.P16 gene is a restrain cancer gene found in recently years, it can reversal control a cell directly at its grow and multiply. Cyclin Dl was found in 1991, it is generally thought cancer gene. PI6 albumen and Cyclin Dl use contest way to connect with CDK4, so to restrain the activity of CDK4, and make the cell stop growing in GO or Gl, inhibit the cell multiply and polarization.Vulvar cancer (VSCC)is a uncommon cancer in gynaecology malignancy, among this vulvar squamous carcinoma is common , about 80 - 90%. VIN is u-sually called vulvar non - representative grow, the incidence of a disease have a increasing tendency in recent years, and it also be believed related with human papilloma virus ( HPV). VNNED was called vulvar innutrition, was regarded as a disease before carcinoma, now only find it will to turn to carcinoma in epidemic , whether their have any same variation in molecule is not known.This test use immunohistochemistry method to check and measure the expression of Cyclin Dl and P 16 albumen in cell cycle in order to find the relation to vulvar carcinoma.Material and Methods1. Clinical material: Cases from the First Hospital of China Medical University were collected between January 1994 and June 2003, including 30 cases of vulvar squamous carcinoma; 40 cases of VNNED, 20 of them were squamous cell hyperplasia, the other 20 were lichen sclerosus of vulva; 30 cases of VIN, 10 VIN I , 10 VIN II , 10 VIN I; 10 cases of normal vulvar skin.2. Immunohistochemistry was employed in this study to detect expression of Cyclin Dl protein (mouse anti - human Cyclin Dl monoclonal antibody, 1:100; ready to use. Maxiam Biotech) and PI6 protein (goat anti -mouse IgG, 1; 100,ready to use, Maxiam Biotech). All procedures were implemented according to the product illustrations. We used PBS instead of primary antibody as negative control.The immunostaining of PI6 and Cyclin Dl was localized to the nucleus and cytoplasm. Statistical evaluation was performed using chi - square to differentiate the rates of different groups. SPSS 10. 0 software was employed to analyze all data.Results1. The expression of PI6 and Cyclin DlThe expression of PI6 protein was mainly in nucleus, in normal vulvar skin positive mainly in fundus cells, in abnormality cells of VIN,in thorn cells of VNNED and also in the cells of carcinoma have the expression of PI6 protein, it is descend gradually.The expression of Cyclin Dl mainly in the nucleus of epithelial cells and carcinoma cells, in normal epithelial is no expressed, in abnormality cells of VIN , in thorn cells of VNNED and also in the cells of carcinoma have the expression of Cyclin Dl, it is increased gradually, and it was in carcinoma cells.2. Positive rate of PI 6 and Cyclin Dl among different groupsP16 protein positive rate was 100% (10/10) in normal vulvar skin, 87. 5%(35/40) in VNNED, 83.3% (25/30) in VIN and 40.0% (12/30) in vulvar squamous carcinoma. The positive rate of PI 6 in normal vulvar skin was higher than that in VIN and vulvar squamous carcinoma, but the difference was not significant; there were significant differences among vulvar squamous carcinoma, normal skin, VNNED, and VIN (P<0.05).The positive rate of Cyclin Dl was 0% (0/10) in normal vulvar skin, 35. 0% (14/40) in VNNED, 43.3% (13/30) in VIN and 63. 3% (19/30) in vulvar squamous carcinoma. There were significant differences of Cyclin Dl expression among normal vulvar skin with vulvar squamous carcinoma, VNNED, and VIN ( P <0. 05) ; there were also significant differences among vulvar carcinoma with VNNED and VIN (P <0. 05) ; but there was no significant difference between VNNED and VIN (P > 0. 05).3. |
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