Experimental Studies On The Treatment Of Chronic Glomerulonephritis With Niaozhuoqing

Objective: To study the therapeutic effect of Niaozhuoqing (NZQ) preparation on experimental rat models of chronic glomerulonephritis (CGN) and to explore the mechanism of the preparation. Methods: two kinds of animal models of CGN were reproduced, one was the minimal change nephropathy (MCN) rat model induced by tail-intravenous injection of Adriamycin and the other was membrane-damaged CGN rat model induced by tail-intravenous injection of cationized bovine serum albumin (C-BSA). After treated with NZQ preparation for 4 or 5 weeks, several measurements were done for 24-hour urinary protein contents, serum protein products of lipid metabolism, blood urea nitrogen (BUN), serum creatinine (SCr), activity superoxide dismutase (SOD) and malondialdehyde (MDA) in serum. In addition blood ratio viscosities were observed and the serum levels of interlenkin-8 (IL-8) and tumor necrosis factor (TNF- a ) were measured in rats with CGN. Also renal histopathological changes were observed by microscope. Results: NZQ given orally had obvious therapeutic effects on rats with CGN. In comparison with the untreated control group, the content of proteinuria in 24 hours, BUN, SCr, MDA, IL-8, TNF- a mean levels and the blood ratio viscosities in NZQ-treated groups were markedly lower (p<0.05, 0.01), serum SOD mean value was significantly higher (p<0.05) and the pathological changes of glomeruli were alleviated. Conclusion: NZQ had shown significant effect on the treatment of CGN by protection of renal function which might involve improvement of high coagulation state,elimination of oxygen free radicals, inhibition of lipid oxygenation, decrease the secretion of inflammatory cytokines including IL-8 and TNF-a , suppression the recruitment of inflammatory cells in the kidney.