Molecular Detection Of Clonal Gene Rearrangement In Non-Hodgkin's Lymphoma

The diagnosis and differential diagnosis of lymphoma was a difficult work all the while. Since the molecular technique was applied to the lymphoma study at the end of 1980', the characters of molecular biology and molecular genetics have become indispensabable to the diagnosis and research on lymphoma.In this project, some molecular biological techniques were applied to study the clinical significance of molecular analysis on non-Hodgkin's lymphoma. Part One Clonal Heavy-Chain Gene Rearrangement Detection of B cell Lymphoma in Paraffin-Embedded TissueObjective To analyze the value of detecting clonal heavy-chain gene rearrangement in B cell lymphoma (B-NHL). Methods The clonality of the third complementary determining region of immunoglobulin heavy-chain (IgHCDR3) gene rearrangement in paraffin-embedded tissue of 23 B-NHL, 7 T cell lymphoma (T-NHL) and 6 benign reactive diseases were investigated by semi-nested polymerase chain reaction(semi-nested PCR), polyacrylamide gel electrophoresis (PAGE) and silver staining. All cases had previously been studied by histology and immunohistochemistry. Results the positive rate of clonal IgHCDR3 rearrangement was 87.0%(20/23)in B-NHL, faulty negative rate was 13.0%(3/23), 1 case with T-NHL showed IgHCDR3 positive, but all 6 cases of benign reactive diseases showed negative, the total faulty positive rate was 7.7%(1/13). Conclusion The result showed that detection of clonal IgHCDR3 may be useful in adjuvant diagnosis and differential diagnosis in B-NHL.