| Objective: To explore the relationship between the inflammation and atherosclerosis disease in diabetic rats. Methods: The models of diabetic rats were made by injecting streptozocin into rat' s abdomen. Wistar rats were divided into four groups : three diabetes groups and control group.At one month , three months and five months,the rats' arterys were collected as experimental material to exam the IL-6 and TNF- α by using immunohistochemistry method, at the same time , the blood samples were collected to exam level of lipid, hCRP,IL-6 and TNF-α by using ELISA. Results: (1)Compared with normal control group, the blood glucose of diabetic rats were highter and had significant differences. There were significant differences between TC, LDL, TG, NEFA, NO of diabetic rats and that of normal rats. The concentration of TC, LDL, NO in diabetes were lower than that of normal rats, and the concentration of TG, NEFA in diabetes were higher than that of normal rats. Higher hCRP,IL-6 were observed in diabetic groups' blood samples, but there were no significant differences . (2) Immunohistochemistry results: There were IL-6 and TNF-α specific deposits in diabetic rats' atherosclerotic wall. The more extracellular deposits were observed in the atherosclerotic intima, compared with the normal one. Conclusion: (1) Without other risk factors of athersclerosis, the diabeticatherosclerosis,but also the hyperglycemia is the risk factor.(2) There were expression of inflammation factors at atherosclerotic wall. It suggested that inflammation factors may be a risk factor of atherosclerosis ;(3) The expression of inflammation especially at atherosclerotic wall,not in blood.It suggested that the inflammation is just in diabetic atherosclerotic wall,and the maybe reason is the easiness of injury at endothelial cells.
|
PDF Full Text Request