Study On A Preparation Of The New Proliposome

a new method of making proliposome had been established and tegafur proliposome had been prepared according to this method .In this thesis choosing silybin,oleanolic acid and herba cistanches as model drug,which were different in property, we studied the applicability and scope of the method, which could help us understand the mechanism of the method and the effort of different drugs.Firstly, single factor design was employed to choose suitable solvent, the quantity of phospholipid and additives, and then every kind of formulas was determined. Secondly, a series of physical and chemical characteristics of proliposome after integrating with water was measured, such as using transmission electron microscope method to observe shape, and laser irradation method to measure size distribution and surface electronic potential; using sephadex column layer analysis method to measure trap efficiency;according to the results of the trap efficiency, some factor of trap efficiency was detected and compared with tegafur proliposome, which was invented by another student in our group. Finally, intestional absorption experiment in vitro of silybin and oleanolic acid was employed to determine the absorption ability of proliposome.The results proved the proliposome of three drugs above can form liposome with particle size well-distributed, and the lipophilic drugs with higher purification can be made into proliposome more easily, furthermore, the proliposome can improve the intestinal absorption and increase bioavailability, which can also prove the application of the method.The study can help to know the scope of the method and indicate the importance of making oleanolic acid and silybin liposome.