The Correlation Of The Expression Of CD11b/CD18 On Neutrophils In Peripheral Blood And The Concentrations Of IL-8 Serum In COPD

Objectives: 1.To investigate the expression of leukocyte adhesion molecules CD11b/CD18 on the surface of neutrophils in peripheral blood and the changes of concentration of IL-8 serum in patients with stable and exacerbated COPD. 2. To evaluate the relationship between two biochemical parameters and COPD in order to explore the role of CD11b/CD18 on the surface of neutrophils and IL-8 serum in the pathogenesis and progress of COPD,and provide reference for the early diagnoses and treatment of COPD. Methods: According to the guideline of COPD 2002 of Chinese Medical Association, forty males with COPD were included randomly in the study and twenty age and sex-matched healthy cases served as control subjects. Twenty patients, including 15 men , 5 women,were in the severe stage of COPD,whose average age is 61.40±8.03;Twenty patients, including 14 men,6 women,were in the stable stage of COPD,whose average age is 61.10±5.90. The pulmonary function were evaluated in all patients. Inclusion criteria for the present study were FEV_1.0<80%,FEV_1.0/FVC<70%, after inhaling bronchodilators. The patients with COPD must have some clinical symptoms and diagnoses of chest radiography . All the patients had not been treated by glucocerticoid, immunosuppression for three months. The patients with cardiovascular disease, cerebrovascular disease, liver and renal disease, connective tissue disease, oncologic disease and other infectious disease were excluded from this study. There were no significant difference in age and sex among three groups. The positive expression rates of CD11b/CD18 on the surface of neutrophils in peripheral blood were analyzed by flow cytometry using monoclonal antibodies. Meanwhile, the concentrations of IL-8 in serum were measured by means of ELISA. Difference in serum IL-8 and the positive expression rate of CD11b/CD18 among three groups were assessd by using one-way analysis of variance and further multiple comparisons were performed with SNK-q test. Furthermore, linear correlations were performed between CD11b/CD18, IL-8 and pulmonary function parameters FEV1.0,FEV1.0/FVC of patients with COPD, respectively. Meanwhile, the correlation between serum IL-8 and the positive expression rate of CD11b/CD18 was investigated. Results: 1.The positive expression rates of CD11b/CD18 on the surface of neutrophils in peripheral blood were 18.99±6.04(%), 31.37±13.18(%) , 50.38±15.93(%) in control subjects, stable COPD and exacerbated COPD patients. Compared with control subjects , patients with exacerbated COPD showed an increased expression ofCD11b/CD18 on the surface of neutrophils in peripheral blood (q=11.29, p<0.01). During the stable stage of the disease, compared with exacerbations, the expression of CD11b/CD18 on the surface of neutrophils in peripheral blood were reduced(q=6.84, p<0.01).However, compared with controls, they were still higher ( q=4.45, p<0.01 ) .2. The serum IL-8 concentrations were 55.51±19.83pg/ml, 100.06±16.09pg/ml, 144.1±29.06pg/ml in control subjects, stable COPD patients and exacerbated COPD patients, respectively. The serum IL-8 concentrations were higher in patients with both stable COPD and exacerbated COPD than those in control subjects(q=10.32,p<0.01;q=19.83, p<0.01). Compared with those in patients with stable COPD, the serum IL-8 concentrations were higher with exacerbated COPD(q=9.51,p<0.01). 3. The linear correlations were found between two biochemical parameters and FEV1.0, FEV1.0/FVC in patients with stable COPD and exacerbated COPD as follows: ①The serum IL-8 levels were correlated negatively with FEV1.0 both in stable and exacerbated COPD patients(r=-0.509,p<0.05;r=-0.496,p<0.05,were correlated negatively with FEV1.0/FVC(r=-0.469,p<0.05;r=-0.464,p<0.05).②The positive expression rates of CD11b/CD18 on the surface of neutrophils in peripheral blood were correlated negatively with FEV1.0 both in stable and exacerbated COPD patients(r=-0.559,p<0.05;r=-0.592,p<0.05 ), were correlated negatively with FEV1.0/FVC(r=-0.512,p<0.05;r=-0.513,p<0.05). 4. Both in exacerbatedan...