The Relationship Between Detection Of Human Papillomavirus DNA By Fluorescence Quantitative Polymerase Chain Reaction And Cervical Carcinoma

Objective: To detect quantitation of human papilloma-virus(HPV) DNA of cervical epithelium tissue in patients with cervical carcinoma or chronic cervicitis, and to explore the relationship between HPV and cervical squamous cell carcinoma or chronic cervicitis and the molecular mechanism of cervical carcinoma.Methods: Fluorescence quantitative polymerase chain reaction(FQ-PCR) was used for the detection of HPV 16 DNA and HPV 18 DNA in 30 cases of cervical carcinoma and 18 cases of tissues near to carcinoma and 30 chronic cervicitis and 30 cases of normal cervical epithelium tissue.Results: Twenty-two out of 30 cervical carcinoma specimens' tissues were confirmed to be HPV-DNA 16 type positive (73.3%). The average copy numbers of DNA template in 16 type positive samples was 4.89×10~5 (1.2×10~3~ 2.41×10~7). Seven out of the specimens' tissues were confirmed to be HPV DNA 18 type positive (23.3%). The average copy numbers of DNA template in 18 type positive samples was 1.54×10~4 (1.06×l0~3~ 5.36×10~5). Both HPV16 DNA and HPV18 DNA were positive in four cases (13.3%). HPV18 DNA was positive, but HPV 16 DNA was negative in 3 cases. HPV 16 DNA was positive, but HPV 18 DNA was negative in 18 cases. The average copy numbers of DNA template in 16 type positive samples was 2.83×l0~6(1.2×10~3~ 2.41×10~7). HPV16 DNA was positive in 9 cases of tissue near to carcinoma accompanying cervical intraepithelial neoplasm Ⅱ -Ⅲ(CIN Ⅱ -Ⅲ) (60%). The average copy numbers of DNA template was 2.77×10~4(1.01×l0~3~ 9.41×10~5). HPV18 DNA was positive in 1 case of tissue near to carcinoma else. Five out of 30 chronic cervicitis tissue HPV16 DNA was positive (16.7%). The average copy numbers of DNA template was 5.85×10~3 (1.06×10~3~ 3.41 ×10~4). Each of them HPV18 DNA was negative. In 30 cases of normal cervical epithelium tissue, HPV DNA was negative. The difference was markedly significant. And the difference of HPV positive rate between tissue near to carcinoma and cervical cancer(stage Ⅰ -Ⅲ) is also significant.Conclusion :This study indicated that the infection of HPV 16,18 is one of the important factors in the development of cervical carcinoma. The quantitation of human papilloma-virus (HPV) 16 DNA is associated with cervical carcinoma. Fluorescence quantitative polymerase chain reaction maybe an effective method in detection of HPV 16,18 infection and early diagnosis of cervical carcinoma.