| Objective: Explore the correlation between the cell-free fetal DNA in maternal blood and the threatened premature delivery.Methods: 30 cases, bearing a male fetus by B-ultrasonic, of premature delivery and the cured threatened premature delivery in the hospital were selected as control group and 30 cases, bearing a male fetus by B-ultrasonic, of normal pregnancy as contrast group. The gestational ages of the two groups have no significant difference. SRY levels in maternal blood were quantified with PCR (QF-PCR), the concentration of fetal DNA with SRY gene therein being measured; the cervix length was measured with JILANTAIMAGEPOINT. The difference of the concentration of fetal DNA and the cervix length between the two groups were compared using variance analysis and Q test.Results: In-group comparison: the fetal DNA concentration of 20 premature delivery cases ranges from 212.3-2632.2/ml, with mean 625.5 ± 52.4; while the other 10 cured premature delivery cases ranges from 146.3-1765.6 copy/ml, with mean 220.4±57.5 copy/ml. Variance analysis and Chi-Square test: X2 = 1.0890, p=0.5983. Q test indicates that the fetal DNA concentration of the threatened premature delivery is significantly higher than that of the contrast group (189.2±43.2 copy/ml), and the premature delivery significantly higher than the threatened premature delivery (at p<0.001), The mean cervix length of premature delivery group is 28. 9±6 .8mm, the threatened premature delivery group 41.8±64mm and the normal pregnancy group 42.2 ±5.8mm. The difference both between premature delivery cases and threatened premature delivery cases and between the premature delivery cases and the normal pregnant cases is very significant at P<0. 01; however, the difference between the normal pregnant cases and the threatened premature delivery cases is not significant at P>0.05. Negative relation was observed between the fetal DNA concentration and the mean cervix length ( r=- 0.676, p<0.01 ) .Conclusion:The concentration of the cell-free fetal DNA in maternal blood is highly correlated with the threatened premature delivery. |
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