| Objective: The proliferation of glomerular mesangial cell(MCs) and accumulation of extracellular matrix(ECM) were nuclear pathological link in the developmental process of various primary glomerulonephritis, for instance mesangioproliferative glomerulonephritis, proliferative endocapillary glomerulonephritis, membranoproliferative glomerulonephritis, and ECM accumulation was also chiefly pathological manifestation in renal fibrosis and in terminal period of glomerulopathy. Inhibiting MCs proliferation and ECM accumulation was key in treating the renal disease. ShenLuoTong(SLT) was verified prescription, which was used in treating the renal disease by ZhaoYuYong professor, and which was obviously effective in clinical and animal experiment. In the light of above mentioned views, by the methods of cell cultivation and serum pharmacology, MCs cultivated in vitro was intervened by the serum containing SLT, then it was observed that MCs proliferation and ECM accumulation was affected by SLT. Objective and significance of the research as follows: 1 Convincing effect would be proved in the aspect of depressing MCs proliferation and decreasing ECM accumulation, and the meanings of combined use of diverse herbs in SLT prescription needed to be clarified. The experimental basis as to treating by activating circulation and relaxing meridian(ACRM) various sorts renal diseases, which have common pathological expression, i.e MCs proliferation and ECM accumulation, was provided, and the directive meaning of ACRM therapy was deepen during the treatment of renal disease, the therapy route of renal disease was widened. 2 The theoretic significance of ShenBingLuoMai hypothesis of traditinoal chinese medicine(TCM) was discussed by means of cell model, for the purpose that the molecular mechanism of LuoBing hypothesis was clarified, and the theoretic guide was offered for ACRM therapy. Methods: The study was made up of four sections. In section one, according to the method provided by ChenYiPu, et al, MCs was cultivated. In section two, the serum containing SLT was prepared in virtue of TongFa scheme. In section three, frozen partial cells were reactivated, then the cells were randomly divided into normal serum group, valsartan group, 1.25% SLT group, 2.5% SLT group and 5% SLT group. Then the situation of cell proliferation was detected after 48h by MTT technique. In section four, frozen cells were reactivated, ECM accumulation model was set up by the stimulation of angiotensin II(Ang II), then the model cell was respectively intervened by normal serum, valsartan, 1.25% SLT, 2.5% SLTand 5% SLT. The amount of IV type collagen(IVCol) and FN (Fibronectin) in upper clear segment of culture fluid by enzyme-labeled immunosorbent assay(ELISA) technique after 48h. Results: 1 Comparison of MCs proliferation in each group In the experiment that MCs proliferation was intervened by SLT, OD value was decreased in SLT of each dosage groups, compared with normal group(P<0.01). Then apparent diminishment was found in SLT of high dosage group compared with SLT of low dosage group(P<0.01). OD value was lower in valsartan group than in normal group(P<0.01), and OD values in SLT of each dosage groups were all lower than in valsartan group, and obvious difference was showed, it was suggested that the effec of SLT in each dosage was superior to valsartan in the aspect of suppresing MCs proliferation.2 Detecting the amount of FN in each group: The amount of FN in SLT of each dosage groups was less than in Ang II model group, and evident difference was demonstated, the amount of FN in 5% SLT group was obviously decreased, compared with 2.5% SLT group and 1.25% SLT group respectively, it was demonstated that certain dosage-effect relationship of inhibiting ECM accumulation by SLT was existed. evident decreasement was showed in valsartan group, compared with Ang II group , nevertheless, the inhibitory action of valsartan was inferior to 5% SLT, it was implyed that certain dosage SLT was superior to valsartan in depressing FN. 3 Testing the amount of IV type... |
PDF Full Text Request