| Object : Numerous studies in recently years demonstrate that glutamine is a conditionly essential amino acid and enhence the synthesis of protein , decrease the catabolism of protein and it can provide nitrogen to synthesize the protein . Glutamine not only provide nitrogen to synthesize nucleinic acid and protein of intestinal mucosal epithelial cell , nephric tubule epithelial cell and lymphocyte , but also is the major fule of these . Intestinal trace is one of the major organs to metabolize glutamine . Glutamine can facilitate the renewal of intestinal mucosal cell , maintain the structure and function of the intestinal trace , so glutamine is regarded as a special nutrition for intestinal mucosal cell . In the state of critical trauma , infection , operation and radiotherapy , the catabolism of glutamine increase obviously because the volume of demand of glutamine improve in organism to lead to the plasma concentration of glutamine decrease notably . The structure and the function of intestinal trace and intestinal mucosal barrier is damaged because the intestinal mucosal absorb glutamine from blood decreasing ,which cause the translocation of the bacterium and endoxin. That which the intestinal mucosa damaged directly or indirectly leads to the gastrointestinal permeatility increasing is the major reason of intestinal bacterium and endoxin translocation . So how to effectly protect the gastrointestinal mucosal barrier in various stress is one of the aims of the scholars all over the world to study . Glutamine is the major fuel for the enterocyte and major energy origin of intestinal mucosal cell and prevent gastrointestinal mucosal cell from atrophying . Although there have had a lot of studies of how to protect the gastrointestinal mucosal in various stress , such as trauma , burn and complex operation ,how the glutamine effect the gastrointestinal mucosa in the period of themothraphy don't be reported in the past . So we design the test to study the principle of how the glutamine effect the gastrointestinal mucosa and find the ways to prevent the intestisal mucosa from damaging in the period of chemothraphy . Methods: Fourty Wistar rats were randomly and averagely divided into four groups: A : control group , B : 5-Fu group , C : 5-Fu + Glycin group , D : 5-Fu + Glutamine group . After the rats were weighted and recorded , the rats in group C was given Glycin , group D glutamine (0.35g/kg each) , group A and group B the same doses of saline solution intragastric administrate respectively from day1 to day6 . Group B,group C and group D were received 5-Fu (100mg/kg) injection introperitoneally on the fourth day respectively tomake the model of gut barrier injury. At the same time, group A received the same doses of saline solution injection introperitoneally. The content of endotoxin in peripheral blood were measured in all groups after rats were weighted and killed on the seventh day. The mesenteric lymph nodes (MLN) and liver were harvested for comparing bacterial translocation incidence among four groups. Ileum and colon tissue were taken for pathologic examination including comparing mucosal thickness, villus height and width and morphology change of ileum and the weight of each group before and after the test. Statistical Analysis System 6.12 (SAS6.12)was used for data of statistical analysis. Analysis of variance and SNK-q test were used for quantitative data analysis, Fisher's least-significant-difference test for qualitative data. P values that were less than 0.05 were considered statistical significance . Results: ①Compared the weight of each group before and after the test : There were not significant difference in group A (230.1±11.76g), groupB (226.7±14.29g), groupC (228.4±15.11g)and groupD(235.80±9.15g)before test (P>0.05) .After test ,there were not significant difference in group A (233.3±10.29g)and group D(234.9±8.84g) (P>0.05) . There were the same between group B(217.4±13.24g) and group C(219.2±17.59g) (P>0.05). However, the value in group A and group D were significant more than group B and group C(P<0.05) . ②The content of endotoxin in peripheral blood ofeach group : No significant difference between group A(0.042±0.010Eu/ml) and group D(0.054±0.010Eu/ml)(P>0.05) , and the same beween group B(0.090±0.013Eu/ml) and group C (0.099±0.020Eu/ml)(P>0.05).The value of group A and group D were significantly lower than that of group B and group C (P<0.05). ③The value of ileum villus height in each group : Group A (391.58±14.18μm) were significantly higher than group B(279.51±20.41μm) ,group C(275.72±21.71μm) and group D(325.09±27.40μm)(P<0.05). Group D were significantly higher than group B and group C (P<0.05) . The value of group B and group C were not significantly different (P>0.05) . ④The value of ileum villus width in each group :Group A (167.06±19.05μm) were significantly wider than group B(121.16±16.47μm),group C(114.54±9.75μm) and group D(149.55±17.81μm)(P<0.05). Group D were significantly wider than group B and group C (P<0.05) . The value of group B and group C were not significantly different (P>0.05) . ⑤T he value of ileum mucosal thickness in each group : Group A (524.00±36.49μm) were significantly thicker than group B(450.26±34.52μm),group C(459.11±23.51μm) and group D(487.01±10.85μm)(P<0.05). Group D were significantly thicker than group B and group C (P<0.05) .The value of group B and group C were not significantly different (P>0.05) . ⑥The value of colon mucosal thickness in each group : Group A (352.98±35.27μm) were significantly thicker than group B(263.29±42.60μm),group... |
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