The Acute Effects Of Omethoate On Malondialdehyde, Superoxide Dismutase And Glutathione Peroxidase And The Role Of Vitamin C In Rats

Objective: To investigate the change of serum malondialdehyde(MDA), superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px) in a level of acute omethoate intoxicated rats and the ameliorating effects of vitamins C.Methods:1. 72 rats were equally assigned to the control group, 0.1 LD50 omethoate group, 0.5 LD50 omethoate group, and 0.5 LD50 omethoate +VitC(200mg/kg) group.2. Sampling and storage: The blood sample were obtained from the heart of the rats at 12h, 24h, and 48h after subcutaneous injection of omethoate. They were centrifuged at 3000 rpm for 10min and stored at -70° C until use. The serum Ache, MDA ,SOD and GSH-Px were determined .3. Atistical analysis: All adats were presented as means and standard deviations. They were compared using analysis of q test by SPSS-PC. A P value of <0.05 was considered significant.Results:1. Serum MDA level increased significantly in 0.1 LD50 and 0.5 LD50 omethoate group compared to the control group (P < 0.05), and increased significantly in 0.5 LD50 group omethoate compared to 0.1 LD50 omethoate group (P<0.05) .but decreased significantly in 0.5 LD50 omethoate +VitC group (P < 0.05).2. The activity of SOD and GSH-Px decreased significantly in 0.1 LD50 0.5 LD50 omethoate group compared to the control group (P < 0.05), decreased significantly in 0.5 LD50 omethoate group compared to 0.1 LD omethoate group (P<0.05).3. The activity of SOD and GSH-Px increased significantly in 0.5 LD50 omethoate +VitC group compared to 0.5 LD50 group omethoate (P < 0.05).conclusion:1. There are increase of lipid peroxidation in acute omethoate intoxicated rats.2. There are decrease of anti-oxidant enzyme activities in acute omethoate intoxicated rats.3. Treatment with vitamins C can reduce lipid peroxidation caused by omethoate.