Study On The Serum Levels Of MDA,SOD,GSH-Px Of Patients With Chronic Respiratory Failure

Objective The aim of this study isâ‘ by determinating the levels of malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxides (GSH-Px)in the serum of patients with chronic respiratory failure before and after treatment,to explore the oxidative stress in patients with chronic respiratory failure and the role of the three markers in the pathophysiologic condition and clinical significance of chronic respiratory failure.â‘¡To analyze the correlation of the levels of MDA,SOD,GSH-Px of the serum and the arterial blood gases in patients with chronic respiratory failure,to investigate the clinical significance of the three markers in the evaluation of the severity and the progression of the disease.Methods The study population consisted of 40 patients with chronic respiratory failure,including 13 patients with typeâ… ,27 patients with typeâ…¡and 25 controls. We measured the concentration of MDA through the method of Thiobarbituric acid-reaction,the activity of GSH-Px by the method of glutathion reductase consumption,the activity of SOD by the method of xanthine oxidase in the serum of all the participants before and after treatment.We obtained the arterial blood gases parameters partial pressure of oxygen in arterial blood(PaO₂),partial pressure of carbon dioxide in arterial blood(PaCO₂).Resultsâ‘ SOD:The activity of SOD in typeâ… respiratory failure(56.53±11.61 U/ml)and typeâ…¡respiratory failure(70.76±13.35 U/ml)were significantly lower than that of controls(86.78±16.03U/ml)(P＜0.01)before treatment.It was significantly higher in typeâ…¡respiratory failure than typeâ… respiratory failure(P＜0.05).The activity of SOD in typeâ… respiratory failure(76.29±7.53U/ml) and typeâ…¡respiratory failure(81.08±13.98U/ml)remained significantly lower compared to controls(92.12±15.15U/ml)(P＜0.01,P＜0.05)after treatment.And there is no significance between typeâ… respiratory failure and typeâ…¡respiratory failure(P＞0.05).â‘¡GSH-Px:The activity of GSH-Px in typeâ… respiratory failure(112.65±18.51)was lower compared to that of typeâ…¡respiratory failure (129.15±18.70)and controls(132.13±23.00)(P＜0.05)before treatment.And there was no significance between typeâ…¡respiratory failure and controls(P＞0.05).There were no significance among the three groups(P＞0.05)after treatment.â‘¢MDA:The concentration of MDA in typeâ… respiratory failure(9.68±0.81nmol/ml)and typeâ…¡respiratory failure(9.20±1.55nmol/ml)were significantly higher compared to that of controls(7.64±1.47nmol/ml)(P＜0.01)before treatment.And there was no significance between typeâ… respiratory failure and typeâ…¡respiratory failure (P＞0.05).There was no significance among the three groups(P＞0.05)after treatment.â‘£PaO₂:The degree of PaO₂ in three groups after treatment are significantly higher than pretherapy(P＜0.01).⑤PaCO₂:The degree of PaCO₂ in typeâ…¡respiratory failure after treatment(49.8±25.90mmHg)was significantly lower than pretherapy(72.88±11.89mmHg)(P＜0.01).â‘¥The degree of PaO₂ showed a significant negative correlation with the concentration of MDA(r=-0.572, P＜0.05),while positive correlation with the activity of SOD(r=0.719,P＜0.05)in typeâ… respiratory failure.⑦The concentration of MDA showed a significant negative correlation with the activity of SOD(r=-0.943,P＜0.01)and GSH-Px(r=-0.355, P＜0.05).Conclusionâ‘ There is obvious oxidative stress in patients with chronic respiratory failure,reflects as the increased level of oxidants and the decreased activity of antioxidants.â‘¡The level of oxidants were decreased and the activity of antioxidants were increased after the treatment of effective therapy.â‘¢There were correlation between the arterial blood gases parameters and the levels of MDA,SOD and GSH-Px in the serum,it was presumed that MDA,SOD and GSH-Px may be useful indexes in evaluating the severity and the degree of the progression in patients with chronic respiratory failure.â‘£It was found that the hypercapnic acidosis in a degree may be decrease the damage of oxidative stress to the patients with chronic respiratory failure.