The Assessment Of Toxic Effect Of Different Concentrations Of Povidone Iodine Made In China On The Rabbits' Cornea

Objective To investigate the toxic effect of different concentrations of povidone iodine made in China on the rabbits' cornealepithelia and endothelia, and to discuss the feasibility and suitable concentrationof PVP-I used as conjunctival sac instillation before eye surgery or after surgery. Methods group A, conjunctival sac instillation of 5.0%PVP-I, assessed by means of specular microscopy and corneal pachymetry; group B, conjunctivalsac instillation of different concentrations of PVP-I, the damage of corneal epithelia is observed after fluorescence dye; group C, the effect on endothelia observed after the injections of 0.05ml of different concentrations of PVP-I into the anterior chamber. Each group is compared to a corresponding control group-0.9%NS. Result Group A, there was no significant difference in the main parameters obtained between 0.5%PVP-I and NS (P>0.05). Group B, severe damage was observed on the cornel epithelia at concentration of 5.0% and 2.5%, but the cornea is clear at concentration of 1.0% and 0.5%. Group C, significant corneal edema was observed at concentration of 2.0% and 1.5%, but there was no significant difference in the main parameters between 1.0% and NS or 0.5% and NS (P>0.05). Conclusion Considering the effect on both corneal epithelia and endothelia, it is safe and feasible to use 0.5% or 1.0% PVP-I as conjunctival sac instillation for antisepsis before eye surgery and as an alternative to postoperative topical antibiotics.