The Study On The Polymorphism Of Fibrinogen Gene 148 C/T And Plasma Fibrinogen Levels In Patients With Coronary Heart Disease

Objective To investigate the frequency of beta-fibrinogen (beta-Fg) gene-148 C/T polymorphisms and their association with plasma fibrinogen levels in patients with coronary heart disease(CHD).Methods The B beta gene Fg -148 C/T polymorphism was identified by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) in 128 patients with CHD and 110 healthy controls matching on age and sex. Prothrombin time assays were performed to measure the plasma fibrinogen levels of all cases.Results The distribution of Fg -148 C/T gene polymorphism was inaccordance with the Hardy-Weinberge quilibrium(P>0.05). The genotype frenquencies of 148 C/T are higher in CHD group than that in control group. The frenquencies of T allele in CHD group(0.309) are higher than that in control group(0.209)(P<0.05). The plasma fibrinogen level in CHD group (3.42+/-0.52 g/L) was significantly higher than that in the controls (2.96+/-0.42g/L) (P<0.01). Plasma fibrinogen levels in CHD patients with T148 alleles (148CT,148TT)were significantly elevated than that in patients without T148 alleles (148CC)(P<0.05).Conclusion the incresesed plasma Fg level is a risk factor of CHD. The beta-fibrinogen gene Fg -148 C/T polymorphism maybe play an important role in the pathogenesis of CHD.