Experimental Research On The Effect Of Extract From Gu-Sui-Bu (Drynaria Fortunei J. Sm) On Osteogenic Differentiation Of Rabbit Bone Marrow Stromal Cells In Vitro

In the traditional Chinese medicine, Gu-Sui-Bu[Drynaria Fortunei(kunze) J. Sm] has been reported as a good enhancer for bone healing. In this experiment, we investigate the effects of this traditional Chinese medicine on osteogenic differentiation of bone marrow stromal cells in vitro. And try to discuss that marrow stromal cells is a kind of potential target cell of Gu-Sui-Bu [Drynaria Fortunei(kunze) J. Sm] or these traditional Chinese medicine that were proved to regulate kidney to promote bone qualities and enhance bone healing.Different concentrations of ethanol extract of Gu-Sui-Bu were added to rabbit marrow stromal cells culture. The effect on osteoblastic differentiation was examined by reserved phase-contrast microscope, scanning electron microscope, histochemistry stains and calcific nodules stains. In the same time, osteoblastic differentiation of these cells cultured by both a condition medium including dexamethasone, sodium β -glycerophosphate and vitamine C and pure medium were examined in same methods.The effective concentrations of Gu-Sui-Bu on osteoblastic differentiation of were 2mg/ml and 0.2mg/ml.The addition of 2mg/ml and 0.2mg/ml respectively to bone marrow stromal cells culture partly expressed osteoblastic phenotype.Firstly these cells induced began to differentiation or transform, then proliferated and secreted, ultimately came into being mineralized matrices in vitro. Histochemical studies...