The Molecular Detection Of Y Chromosome Microdeletions In Idiopathic Azoospermia And Oligozoospermia Patients

Objective To found the molecular detection methods of the Y chromsome microdeletions in patients with idiopathic ozoospermia and oligozoospermia. By using the methods to compare the patients of idiopathic ozoospermia and oligozoospermia and normal fertility men to validate the feasibility of the methods, and also to confirm the microdeletion rate of these patients. Studied the relations between azoospermia factor (AZF) with the patients and the distribution rule of microdeletions of AZF in these patients and to evaluate on the patients who can be applied ICSI and wether they must process prenatal diagnosis after ICSI.Methods By using multiplex PCR technique established moleculor detection methods for Y chromosome microdeletions with 11 STS in AZFa, b, c and d. 68 patients with idiopathic ozoospermia and 57 with oligozoospermia and 45 normal fertility men were detected. Results 1. Established four multiplex PCR methods A, B,C and D, with A, B, C was four and D was three. 2. The microdeletion rate of patients with idiopathic ozoospermia and oligozoospermia was 15.2%(19/125). No microdeletions were found in normal controls(0/45). There were significient difference after statistics compare ( x~2= 7.701,P=0. 006<0. Oo ) . 3. There were 10 in azaoospermia and 9 in oligozoospermia patients with microdeletions, and the microdeletion rates were 14.7% (10/68)and 15.8% (9/57)respectively. There were no significient difference after statistics compare (x2= 0.028, P=0. 876>0. 05) .4. In azaoospermia patients, there were 2 (2/10) with AZFa and 2(2/10) with AZFb alone microdeletions,2 were AZFa+b united microdeletions, AZFb+c, AZFa+b+d, AZFb+c+d and AZFa+b+c were one respectively with microdeletions ,no deletions were detected with AZFc or with AZFd alone or with AZFc+d. In oligozoospermia patients, there were 2(2/9) with AZFb or AZFc or AZFd alone microdeletions respectively , and 2 were AZFb+c+d united microdeletions, 1 was AZFc+d united microdeletions ,no deletions were detected in AZFa.Conclusion 1. The microdeletions of Y chromosome have relations with idiopathic azoospermia and oligozoospermia patients. The microdeletion rate was 15. 2% in idiopathic infertilities. The rate was 14.7% in azoospermia and was 15.8% in oligozoospermia patients respectively.2. The distributions of Y chromosome microdeletions have relations with idiopathic azoospermia and oligozoospermia patients. The azoospermia patients mainly expressed AZFa or AZFb alone...