Study Of Y Chromosome Azoospermia Factor Microdeletions In Patients With Idiopathic Azoospermia In Guangxi Province

Objective:To evaluate the relationship between male idiopathic azoospermia in Guangxi province and Y chromosome azoospermia factor microdeletions.To provide some scientific theory basis for clinic detection and set up the method screening of Y chromosome azoospermia factor microdeletions.Materials and Methods:Forty-eight men with idiopathic azoospermia and twenty normal men controls were included in this study.With multiplex polymerase chain reaction technique(PCR),an analysis of 6 sequence tag site(STS)in AZFa(sY84 sY86),AZFb(sY127 sY134)and AZFc(sY254 sY255) microdeletion of Y chromosome were performed.Results:Y chromosome microdeletion was found in 9(18.75%)of 48 idiopathic azoospermia patients.No microdeletion was found in 20 controls. There was significant difference of deletion incidences between diopathic azoospermia and healthy controls.Among the 9 cases,6(12.5%)deletions were in AZFc,2(4.17%)deletions were in AZFb and 1(2.08%)deletion was in both AZFc and AZFb.No microdeletion was found in AZFa in this study.Conclusion:Microdeletion on AZF gene is a major genetic factors leading to idiopathic azoospermia in Guangxi province.The rate of microdeletion involving AZFc region of Y chromosome is highest in idiopathic azoospermia men.The multiplex polymerase chain reaction technique(PCR)in this study is an easy and reliable method for detecting microdeletions on the Y chromosome.