Expression Of COX-2 In Ovarian Cancer Of The Nude Mice And Inhibitory Effects Of COX-2 Inhibitor On Growth Of Ovarian Cancer

Objective To study the expression of cyclooxygenase-2 in the transplanted ovarian cancer of the nude mice and evaluate the effects of the selective COX-2 inhibitor in inducing proliferation on human ovarian cancer HO-8910 cell line, and to observe the effect of on the transplanted ovarian cancer of the nude mice in vivo. Methods The inhibitory effects of selective COX-2 inhibitor on the growth of HO-8910 were studied in vitro by MTT method. Meanwhile, the inhibitory of tumor growth in transplanted ovarian cancer of the nude mice treated by selective COX-2 inhibitor were determined in vivo. The expression of COX-2 and PCNA in transplanted ovarian cancer tissue of the nude mice was determined by immunohisochemisty. The expression of COX-2 in transplanted ovarian cancer tissue of the nude mice and the inhibitory effects of selective COX-2 inhibitor on the transplanted ovarian cancer tissue were studied by RT-PCR.Results (1) The expression of COX-2 determined by immunohisochemisty was positive in 3 and strongly positive in 7 of the 10 transplanted ovarian cancer tissue. Meanwhile, the expression of PCNA determined by immunohisochemisty was positive in 2 and strongly positive in 8 of the 10 transplanted ovarian cancer tissue. The expression of COX-2 in transplanted ovarian cancer tissue was strongly down-regulated by thetreatment of selective COX-2 inhibitor(P<0. 05). (2) The expression of COX-2 in transplanted ovarian cancer tissue determined by RT-PCR was also down-regulated by the treatment of selective COX-2 inhibitor(P<0. 01) . (3) MTT study showed that selective COX-2 inhibitor produced a dose-dependent and a time-dependent inhibition of H0-8910 proliferation. Conclusions COX-2 expresses in the transplanted ovarian cancer tissue of the nude mice. Selective COX-2 inhibitor can significantly inhibit ovarian cancer in vivo and in vitro.