The Different Expression Of PTEN Gene In Human Glioma Cell Line SWO38 And Its Two Sublines

Objective To investigate the different expression of PTEN gene in human glioma cell line SWO38 and its two sublines SWOZ1 and SWOZ2. To explore the molecular mechanism of tumor heterogeneity of human glioma.Methods (1) PTEN gene in glioma cell line SWO38 and its two sublines SWOZ1and SWOZ2 was amplified by RT-PCR. The expression level of PTEN gene was examined by luminosity of electrophoretic band. (2) All samples were screened for mutations in PTEN gene by PCR-SSCP analysis. (3) PTEN gene amplified from the glioma cell line SWO38 and its two sublines was sequenced and compared with the international nucleotide database using BLAST to inspect whether there presented mutation and difference in PTEN gene among the three glioma cell lines.Results (1) PTEN gene expressed in the glioma cell line SWO38 and its twosublines SWOZ1 and SWOZ2, and the level of the expression in three cell lines was different. The gene expression of PTEN was the highest in SWO38, the second in SWOZ2 and the lowest in SWOZ1. (2) We did not find mutations of PTEN gene in three glioma cell lines by PCR-SSCP analysis. (3) The sequences of PTEN gene amplified from the three cell lines were similar in length, nucleotide constitution and alignment. This sequence completely matched with four sequences existed in the international nucleotide database by BLAST. The score was the highest and the expect was the lowest in all of matched alignments, Identities = 1212/1212 (100%), Score = 2403 bits, Expect = 0.0. All of the four sequences were mRNA sequence of wild-type PTEN which contains 1209bp whole coding-sequence. We found that the amino acid constitution between the sequenced PTEN gene and wild-type PTEN were identity by ORF finder.Conclusion (1) There is a definite difference about expression of PTEN mRNAin the glioma cell line SWO38 and its two sublines SWOZ1 and SWOZ2. It suggested that these different gene expressions play an important role in tumor heterogeneity of glioma and relate to the different biological characters of those two sublines. (2) PTENcoding-sequence in SWO38, SWOZ1 and SWOZ2 were completely identical, this indicated that the three cell lines hold one of the aspect of identical hereditary background. (3) The mutation of PTEN mRNA was not detected in SWO38, SWOZ1 and SW0Z2. They held the wild-type PTEN. (4) These two sublines SWOZ1 and SW0Z2 are patent as an ideal model in study of the molecular mechanism of the heterogeneity in glioma.