Detection Of CD44v8-10/CD44v10 Ratio And Surfactant Protein-A Gene In The Cells From Ascites And Pleural Effusion For The Diagnosis Of Lung Adenocarcinoma

Objective To analyse the value of CD44v8-10/CD44v10 ratio and Surfactant Protein-A gene in diagnosis and differential diagnosis of malignant or benign ascites and pleural effusion.Methods (1)Competitive reverse transcription-polymerase chain reaction (Competitive RT-PCR) was performed to amplify CD44v8-10 and CD44v10 in pleural effusion and ascites , Gel-Image Analysis System was used to assay expression quantity of CD44v8-10 and CD44v10 respectively.Calculating the ratio of CD44v8-10/CD44v10 in malignant group and comparing with controls,to build a accurate molecularobiological indicator used in verdicting of malignant or benign ascites and malignant or benign pleural effusion.(2)To analyse the minimal range of RT-PCR,culturing the cell line of lung adenocarcinoma ofGLC-82,and blending it with leukocytes cell in proportional to test it.(3)Use SP-A gene to confirm the oringin of lung cancer, toand validate the RT-PCR product of SP-A with Southern blot.Results (1) The ratio of CD44v8-10/CD44v10 in malignant ascites and pleural effusion was over 1.00 (3.39 ± 2.36/3.62 ± 2.06) compared with under 1.00 (0.55 ± 0.50/0.48 ± 0.29) in benign control , so the cutoff point of CD44v8-10/CD44v10 between malignant and benign lesion was 1.00(P<0.0001);(2) Diagnosis pleural effusion and ascites induced by lung adenocarcinoma with CD44v8-10/CD44v10 ratio and SP-A gene, specificity and sensitivity.Conclusions (1)The ratio of CD44v8-10/CD44v10 was a very fine indicator to verdict malignance or benign of ascites and pleural effusion. (2)SP-A may be a useful indicator to verify pleural effusion induced by lung adenocarcinoma.(3 )The combination of these two tumor markers can enhance the sensitivity further.