The Expression Of MCP-1 And_RANTES In_Malignant Pleural Effusion And Tuberculous Pleural Effuslon

Objective:to compare the expression of chemotactic cytokines in pleural effusions monocyte chemoattractant protein-1(MCP-1)and regulated on activation,normal,T-cell expressed and secreted(RANTES)levels in malignant pleural effusion and tuberculous Pleural effusion.Methods:MCP-1and RANTES were measured by enzyme linked immunosorbent assay(ELISA)in 72 patients with exudates,including 37 patients with tuberculous pleural effusion and 35 Patients with malignant Pleural effusion,as well as their serum samples were measured.After the first successful thoracentesis Of Pleural fluid,a specimen was subjected to routine biochemical analysis including tests for total protein,and lactate dehydrogenase.For the cytokine measurement,Pleural fluid was immediately centrifuged and kept at-70℃until the final Procedure.In addition,blood was drawn for serum analysis and Processed in the same Way as the Pleurat fluid.,we selected 20 healthy People acting as control group.Results:The level of MCP-1 in Pleural fluid was significantly higher In tuberculous group(2211.884士263.226pg/ml)than in malignantgroup(1894.059士 513.289pg/ml)(P＜0.05);Simutanously,the level of MCP-1 in serum was significantly higher In tuberculous group(481.885士258.511pg/ml)than in malignantgroup(51.040士36.840pg/ml)(P＜0.01).in control group,The level of MCP-1 is(57.319士29.259)ng/ml.There Were statistic significant differences between control group and tuberculous group(P＜0.01).In both tuberculous group and malignantgroup,the mean value of the MCP-1 was significantly higher in Pleural fluid than that in serum.2211.884士263.226pg/ml in tuberculous,481.885士258.511pg/ml were in serum,P＜0.05;1894.059士513.289pg/ml in malignant,51.040士36.840pg/ml inserum,P＜0.05).The level of RANTES in Pleural fluid was significantly higher In tuberculous group(302.742士140.753 pg/ml)than in malignant group(161.591士86.439 pg/ml, P＜0.01);The level of RANTES in serum(105.797士68.346pg/ml)was significantly higher In tuberculous than malignant(55.228士26.637pg/ml)(P＜0.01).in control group,The level of RANTES is(96.897士59.817)ng/ml.There Were no statistic significant differences between control group and tuberculous group.The mean values of the RANTES measured in both Pleural fluid were significantly higher than that in serum in Patients(302.742士140.753 pg/ml in tuberculous,105.797士68.346pg/ml in serum,P＜0.05;161.591士86.439 pg/ml in malignant,55.228士26.637pg/ml in serum,P＜0.05).No significant Difference among the three types of lung cancer were found in Both Pleural fluid samples and serum samples(P＞005).conclusions:In summary,(1)MCP-1 and RANTES are significantly higher in tuberculous group than in malignant group.MCP-1 and RANTES in the Pleural fluid could be useful as a complementary marker in the differential diagnosis of the two most common types of exudates(tuberculous,and malignant).(2)MCP-1 and RANTES are significantly higher in exudates than serum.That suggest that there is local chemokines Production in exudative Pleural effusions. (3)In tuberculous group,the contents of MCP-1 in serum were higher than control group,the serum levels of RANTES Were no statistic significant differences between control group and tuberculous group.(4)The contents of MCP-1 and RANTES were not correlated with Pathologic type of lung cancer.