Study Of Association Among The Expression Of PPARγ,PTEN,P27～(kip1) In Endometriol Carcinoma

Objective: Study the expression PPAR γ , PTEN, P27^kipl in endometrial carcinoma, to correlate the proteins expression with histopathology and clinical parametes, and whether they have some relationship with estrogen . Methods: Sixty endometrial carcinoma patients , forty hyperplasia of endometrium and ten normal endometrium paraffin sections were investigated immunohisto-chemically for PPAR γ , PTEN and P27^kipl proteins using the biotinstreptavidin-peroxidase (SP) method. Statistical methods were used to analyze the correlation between (1). expression status of each protein and patients , clinical, histopathologic features; (2)expression status of PPAR γ , PTEN and P27^kipl.Results: (1). In endometrial simple hyperplasia and endometrial complex hyperplasia , the rate of PPAR γ protein positive expression were 10% and 13.33%, it was lower than in atypical hyperplasia of endometrium(33.33%) and was lower significantly than in carcinoma(51.66%); the rate of PTEN protein positive expression were 90% and 86.67% ; and P27^kipl proteinpositive expression were 90% and 80%, they were higher than in atypical hyperplasia of endometrium(60% and 66.67%/' < 0.05) and were higher significantly than in carcinoma(55% and 48.335, P < 0.01).(2). The expression of PPAR y were negatively correlated with histological grade and FIGO tumors stage( P < 0. 05). , While PTEN and P27kipl was positively correlated with histological grade > FIGO tumors stage and muscles invasion (P < 0. 05).(3).There was no significant ralation between PPAR y and PTEN, PPAR Y and P27kipl, but PTEN was positively correlation with P27kipl in endometrial carcinoma ( r - 0. 636 , P < 0. 01).(4). The positive rate of PPAR y .. PTE^ P27kipl protein expression in estrogen-dependent endometrial carcinoma was 61.36%> 63.18%^ 56.82%, significantly higher than that in estrogen-independent endometrial carcinoma(25.00%> 31.35%> 25.00%) , (P<0. 05). Conclusions:(1). PPAR Y > PTE^ P27kipl may play an important role in the occurrence,development and progression of endometrial carcinoma.(2). These proteins may become newly hopeful prognostic factors for endometrial carcinoma.(3). No correlation was found between PPAR Y and PTE^ PPAR Y and P27kipl positive expression. The expression of PTEN was strongly associated with the expression of abnormal P27kipl. (4). PPAR Y <. PTEN . P27kipl may play a role in the pathway of carcinogenesis of type I endometrial carcinoma. The prognosis of in type II is worse than that of in type I .