| Objective Expression of P53,P16,PTEN,Ki-67,and P63 were detected in endometrial adenocarcinoma,and to analyze its correlation with pathological features and prognosis of endometrial adenocarcinoma,and to study the expression relevance of these genes in endometrial adenocarcinoma.Combining detection of expressions of them to reflect the development and progression of endometrial adenocarcinoma,to evaluate the degree of malignancy and prognosis of endometrial adenocarcinoma,to provide experimental evidence for the precise treatment of endometrial adenocarcinoma.Methods The paraffin embedded specimens of 782 endometrial adenocarcinoma patients who sought medical advice at Xijing Hospital from 2001-2015 were included.The first period were 661 cases,Ⅱ period were 88 cases,Ⅲ period were 30 cases,Ⅳ period were 3 cases,depth of myometrial invasion:360 cases for <1/2,422 cases for≥1/2;histological differentiation: well-differentiated were 488 cases,moderately differentiated were 236 cases,poorly differentiated were 58 cases;Vascular tumorthrombus formation were 29 cases.Follow up by telephone and return to the hospital review,recurrence were 69 cases and death were 54 cases.Expression of P53,P16,PTEN,Ki-67 and P63 were detected by immunohistochemical SP method respectively in the 782 cases of endometrial adenocarcinoma.To analyze the relationship between the clinical pathological characteristics and clinical prognosis of endometrial adenocarcinoma and the expression of these genes.Data were analyzed using SPSS 19.0 software,count data using x2 test or Fisher’s exact test,correlation analysis using Spearman rank correlation analysis,P < 0.05 showed that there was a statistically significant difference.Results 1.P53 was mainly localized in cell nucleus,brown yellow particles.The positive expression rates of P53 was 61.9% in endometrial adenocarcinoma.The positive expression of P53 were 58.5%,86.4%,70.0%,0%in endometrial adenocarcinoma ofⅠ-Ⅳ period.The positive expression rate in II period and III period was significantly higher than that in the first period,and the difference was statistically significant(p=0.000);The positive expression of P53 were 8.6%,61.9%,89.7% in endometrial adenocarcinoma of G1,G2,G3 period.The expression of P53 was related to histologic classification(P<0.05=.The positive expression of P53 were 59.7%,63.7%%in <1/2 and ≥1/2 of myometrial invasion of endometrial adenocarcinoma.No relation was observed between P53 expression and myometrial invasion.The positive expression of P53 were 100%,60.4%in vascular tumor thrombus and no vascular tumor thrombus of endometrial carcinoma.The expression of P53 was related to vascular tumor thrombus(P<0.05=.The positive expression of P53 were 52.2%,62.6%in recurrence and no recurrence of endometrial adenocarcinoma.No relation was observed between P53 expression and recurrence.The positive expression of P53 were 38.9%,63.6% in death and no death of endometrial adenocarcinoma.The expression of P53 was related to death(P<0.05=.2.P16 was mainly localized in cell cytosol and nucleus,brown yellow particles.The positive expression rates of P16 was 76.2% in endometrial adenocarcinoma,The positive expression of P16 were 76.1%,72.7%,86.7%,100.0%in endometrial adenocarcinoma ofⅠ-Ⅳ period.No relation was observed between P16 expression and clinical stage.The positive expression of P16 were 76.6%,79.7%,58.6% in endometrial adenocarcinoma of G1,G2,G3 period.The expression of P16 was related to histologic classification(P<0.05=.The positive expression of P16 were 78.3%,74.4%in <1/2 and ≥1/2 of myometrial invasion.No relation was observed between P16 expression and myometrial invasion.The positive expression of P16 were 58.6%,76.9% in vascular tumor thrombus and no vascular tumor thrombus of endometrial adenocarcinoma.The expression of P16 was related to vascular tumor thrombus(P<0.05).The positive expression of P16 were 94.2%,74.9%in recurrence and no recurrence of endometrial adenocarcinoma.The expression of P16 was related to recurrence(P<0.05).The positive expression of P16 were 100.0%,74.8%in death and no death of endometrial adenocarcinoma.The expression of P16 was related to death(P<0.05=.3.PTEN was mainly localized in cell nucleus,brown yellow particles.The positive expression rates of PTEN was 10.9% in endometrial adenocarcinoma,The positive expression of PTEN were 10.4%,14.8%,10.0%,0% in endometrial adenocarcinoma of Ⅰ-Ⅳ period.No relation was observed between PTEN expression and clinical stage.The positive expression of PTEN were 9.4%,16.5%,0%in endometrial carcinoma of G1,G2,G3 period.The expression of PTEN was related to histologic classification(P<0.05=.The positive expression of PTEN were 8.6%,12.8%in <1/2 and ≥1/2 of myometrial invasion of endometrial adenocarcinoma.No relation was observed between PTEN expression and myometrial invasion.The positive expression of PTEN were 44.8%,9.6%in vascular tumor thrombus and no vascular tumor thrombus of endometrial adenocarcinoma.The expression of PTEN was related to vascular tumor thrombus(P<0.05 =.The positive expression of PTEN were 17.4%,10.4% in recurrence and no recurrence of endometrial adenocarcinoma.No relation was observed between PTEN expression and recurrence.The positive expression of PTEN were 22.2%,10.1% in death and no death of endometrial carcinoma.The expression of PTEN was related to death(P<0.05=.4.Ki-67 was mainly localized in cell nucleus,brown or brown yellow particles.Thepositive expression rates of Ki-67 was 54.2% in endometrial adenocarcinoma,The positive expression of Ki-67 were 53.9%,44.3%,86.7%,100.0% in endometrial adenocarcinoma of Ⅰ-Ⅳ period.The positive expression rate III period and Ⅵ period was significantly higher than that in the first and second period.The expression of Ki-67 was related to clinical stage(P<0.05=.The positive.The expression of Ki-67 was related to histologic classification(P < 0.05 =.The positive expression of Ki-67 were 44.7%,62.3% in <1/2 and ≥1/2 of myometrial invasion of endometrial adenocarcinoma.The expression of Ki-67 was related to myometrial invasion(P<0.05).The positive expression of Ki-67 were 55.2%,54.2% in vascular tumor thrombus and no vascular tumor thrombus of endometrial adenocarcinoma.No relation was observed between Ki-67 expression and vascular tumor thrombus.The positive expression of Ki-67 were 89.9%,51.1% in recurrence and no recurrence of endometrial adenocarcinoma.The expression of Ki-67 was related to recurrence.The positive expression of Ki-67 were 94.4%,51.7% in death and no death of endometrial carcinoma.The expression of Ki-67 was related to death(P<0.05=.5.P63 was mainly localized in cell nucleus,brown or brown yellow particles.The positive expression rates of P63 was 19.6% in endometrial adenocarcinoma.The positive expression of P63 were 19.1%,18.2%,36.7%,0% in endometrial adenocarcinoma of Ⅰ-Ⅳ period.No relation was observed between P63 expression and clinical stage.The positive expression of P63 were 20.1%,23.3%,0 %in endometrial carcinoma of G1,G2,G3 period.The expression of P63 was related to histologic classification(P<0.05).The positive expression of P63 were 19.7%,19.4% in <1/2 and ≥1/2of myometrial invasion of endometrial adenocarcinoma.The expression of P63 was related to myometrial invasion(P<0.05).The positive expression of P63 were 58.6%,18.1% in vascular tumor thrombus and no vascular tumor thrombus of endometrial adenocarcinoma.The expression of P63 was related to vascular tumor thrombus(P<0.05).The positive expression of P63 were 29.0%,18.6% in recurrence and no recurrence of endometrial carcinoma.The expression of P63 was related to recurrence(P < 0.05).The positive expression of P63 were 22.2%,19.6%in death and no death of endometrialadenocarcinoma.No relation was observed between P63 expression and death.6.Expression of P53 was positively correlated with expression of P16,Ki-67 and P63.The correlation coefficient were 0.087,0.103,0.128 respectively.Expression of P16 was positively correlated with expression of PTEN,Ki-67 and P63.The correlation coefficient were 0.079,0.071,0.245 respectively.Expression of PTEN was positively correlated with expression of P63.The correlation coefficient was 0.242.Conclusions 1.The expression of P53 was related to clinical stage,histologic classification,vascular tumor thrombus and death.No relation was observed between P53 expression and myometrial invasion,recurrence.The positive expression rate was related to mortality rate and prognosis,the expression of wild-type P53 may be related to the degree of malignancy,metastasis and prognosis of endometrial adenocarcinoma.it could be used as a marker for evaluating the degree of malignancy and prognosis.2.Expression of P16 was related to histologic classification,vascular tumor thrombus,recurrence and death.No relation was observed between P16 expression and clinical stage,myometrial invasion.The positive expression rate was related to mortality rate and prognosis,the expression of P16 may be related to the degree of malignancy,metastasis and prognosis of endometrial adenocarcinoma.3.The expression of PTEN was related to histologic classification,vascular tumor thrombus and death.No relation was observed between PTEN expression and clinical stage,myometrial invasion,recurrence.The positive expression rate was related to mortality rate and prognosis,the expression of PTEN may be related to the degree of malignancy,metastasis and prognosis of endometrial adenocarcinoma.4.The expression of Ki-67 was related to clinical stage,muscle invasion,histologic classification,recurrence and death.No relation was observed between Ki-67 expression and vascular tumor thrombus.The positive expression rate was related to mortality rate and prognosis,the expression of PTEN may be related to the degree of malignancy,incursion and prognosis of endometrial adenocarcinoma.5.The expression of P63 was related to histologic classification,vascular tumor thrombusand recurrence.No relation was observed between P63 expression and clinical stage,myometrial invasion,death.The positive expression rate was related to mortality rate and prognosis,the expression of P63 may be related to the degree of malignancy,metastasis and prognosis of endometrial adenocarcinoma.6 P53,P16,PTEN,Ki-67 and P63 have significant impacts on the development of endometrial carcinoma.The expressions of combined detection of them can be used as an important indicator to reflect progression of endometrial carcinoma.It is also a necessary basis for evaluating the degree of malignancy and prognosis.It is also to provide experimental evidence for the precise treatment of endometrial adenocarcinoma. |
PDF Full Text Request