The Effect Of Apoptosis And Proliferation In Hyperplastic Scar And Chronic Ischemic Ulcer Of Rabbit Ears

Objective To observe the effect of rhEGF on chronic ischemic ulcer wound healing of rabbit ears, and to further explore the effect of apoptosis and proliferation in hyperplastic scar and ischemic ulcer of rabbit ears.Methods Establish the animal model of chronic ischemic ulcer wound on rabbit ears. Thirty-eight rabbits were divided randomly into 3 groups as follows:(1)normal control(n=2); (2)injuride control group B(n=36); (3)rhEGF group A(n=36). Establish the animal model of hypertrophic scar on rabbit ears. Eighteen rabbits were divided 2 groups as follows:(4) hypertrophic scar group D(n=18); (5) rhEGF group C(n=18). The immunohistochemistry staining technique was used to detect the expression of PCNA, c-myc and caspase-3.Result There are significant different between the speed of the experimental groups(A and B groups) and that of the control groups, the former is more faster , the rate of healing is rised. PCNA, c-myc and caspase-3 were expressed in all samples. The expressive quantity of PCNA and c-myc in hypertrophic scar were higher than chronic ischemic ulcer and normal skins(p<0.01). The expressive quantity of caspase-3 in normal skins and chronic ischemic ulcer were higher than hypertrophic scar. rhEGF can significantly increased the expression of PCAN, c-myc and caspase-3 in chronic ischemic ulcer. The expression proliferation and apoptosis of fibroblasts showed a relationship with the changes in PCNA, c-myc and caspase-3.Conclusion Exogenous rhEGF showed effects on proliferation of fibroblasts. Proliferation and apoptosis of fibroblasts are important process during tissue repairing. C-myc and caspase protein products may be related to the pathogenesis of the hypertrophic scar.