| Objective: To study the significance of detection of Tenascin-c mRNA in exfoliated urothelial cells for the diagnosis of bladder cancer. Methods: The fresh urine voided firstly in the morning were collected from 32 cases of bladder cancer and 22 patients with benign urogenital diseases and 5 healthy volunteers. Tenascin-c mRNA expression was studied by mean of reverse transcriptase polymerase chain reaction(RT-PCR) in urine exfoliated cells. The urinary cytology analysis was carried out simultaneously. Results: Tenascin-c mRNA from exfoliated urothelial cells has lower specificity (59.3% )than that of cytology analysis (p<0.05 ) , but its sensitivity is 81.3 % which is signicantly higher than that of cytology examinnation (37.5%) (p<0.05). A part of exfoliated urothelial cells from bladder benign lesions may have the same expression intensity of Tenascin-c mRNA as grade I bladder cancer (P>0.05 ), but its significantly lesser intensity than that of grade II III bladder cancer (P<0.05) , There were significant different positive expression rates of Tenascin-c mRNA in urine exfoliated cells from the bladder cancer patients with differrent grades or clinicopathological stages (p<0.05). Conclusions: RT-PCR assay to detect Tenascin-c mRNA in urine exfoliated cells is an extremely sensitive method for the early diagnosis , postoperative inspection of bladder cancer and helps to identificate the grade and stage of bladder cancer,its would become a new method in identification and monitoring of patients with bladder cancer.
|
PDF Full Text Request