Study Of The Protective Effect Of Taurine On Cultured Neurons Toxicity Induced By Manganese In Vitro

Objective: To explore the mechanisms of taurine prevent neurotoxicity mediated by manganese in vitro on the primary cultivated cerebral cortical neurons from neonatal Wistar rats . Methods: neurons were prepared from Cerebral cortical of neonatal Wistar rats within 24 hours. Untill the neurons was optimal about 7 days cultivating in vitro, the assayes began. Grouping: Control(cultivated regulary); Manganese (Mn), The level designed for assay were high, middle and low, 1.0mM, 0.6mM and 0.2mM respectively;Taurine(Tau), Three levels were 4.5mM, 1.5mM and 0.5mM; Then, (Tau+Mn) is 9 dosage-groups based on Factorial experimental design. All goups were terminated and prepared for detections after treatment 24 hours. Detecting indexes as flowing: the morphological changes of neurons, the apoptotic morphology and ratio, DNA damage of neurons and the free Ca2+ concentration in cytoplastic. All the results were analysised with SPSS statistical microsoftware. Result: (1)Tau prevented neurons against Mn toxicity of low and middle concentrations. The ideal conditions can reach upon 4. 5 mM Tau added just, nearly no apoptosis cells could be detected. (2)The percentages of apoptosis in (Tau+Mn)together were lower than those in Mn just , being concentration-dependent relations with Taurine levels. (3)Mn induced DNA damage of neurons with dose-depedent, The higher of Mn concentration, the lengther of the comet tail and the higher percent comet-like cells. Tau incubated could weaken significantly the lesions of Mn towards DNA on the degree. (4) Mn increases free Ca2+ in neurons , Tau treatment could decline significantly the cytoplastic Ca2+ of neurons induced by Mn. Conlusions:Above results suggest: the main mechanisms of Tau preventing cytoxicity against Mn were of declining Mn-induced apoptotic effects, protecting DNA damage and declining free Ca2+ concentrtion in cytoplastic of neurons.