The Experimental Research On ERK1/2 In Mediating The Protection Of Taurine On Hippocampal Neuronal Cells' Injury Induced By Manganese

Objective: By applying the primary culture method on hippocampal neurons, this paper discusses the mechanism of ERK1 / 2 in mediating the protection of taurine on hippocampal neuronal cells in manganese oxidation injury.Methods:Take the hippocampal neurons from the brain hippocampus of SPF Wistar rats which time of birth are less than 24h's. Then put the hippocampal neurons in primary culture till the optimum state.The hippocampal neurons used culture can be divided to several groups as follows:①the control group;②control group taurine (Tau: 4mmol / L);③low concentration of Mn group (Mn: 0.2mmol / L);④medium concentration of Mn group (Mn: 0.8mmol / L);⑤high concentration of Mn group (Mn: 1.2mmol / L);⑥low concentrations of the intervention group (Tau: 4mmol / L, Mn: 0.2mmol / L);⑦medium concentration intervention group(Tau: 4mmol / L, Mn: 0.8mmol / L);⑧high concentration of the intervention group (Tau: 4mmol / L, Mn: 1.2mmol / L).Terminated the culture of each group after 24 hours addressed by treatment factors. Use ordinary optical microscopet to observe and carry out the follow testing: MTT tested for the living rate of hippocampal neurons, Fluorescence detection for intracellular reactive oxygen species generation and increment, Western Blot Detection for ERK1 /2 protein expression, real-time fluorescence quantitative PCR detection for ERK1 / 2 protein mRNA relative expression.Results:①Manganese can cause the morphological changes of hippocampal neurons. And with the concentration increasing,the change can be amplified. The cells form of the control group with intervention was more complete and have more viable cells;②MTT result shows that after damaged by manganese , the viability of hippocampal neurons decreased significantly. There was significant difference (P <0.01) comparing with the control group and taurine control group. When using taurine to intervene, low, medium and high concentrations of Mn groups' activities have significant differences(P <0.01) compared with the corresponding concentration contrast groups. Their activities were better than those exposed to manganese groups;③ROS test shows that comparing with the control group and taurine control groups, the ROS of each adding manganese group increased significantly. The comparison of control group and taurine control group was not statistically significant. The ROS of taurine control groups decreased. There were significant differences(P<0.01)compared with the same concentration of manganese groups. 4 time points measured ROS levels were significantly different,F=18.107,P<0.01;④there was no difference of ERK1/2 protein expression between among low concentration of manganese intervention group,control group and taurine control group. The ERK1/2 protein expression of low,medium concentration manganese groups were lower than those corresponding to the same concentration of manganese in the intervention groups; When come to the high concentration of manganese group and the same concentration of manganese in the intervention group's, ERK1/2 protein expressions have no difference.⑤there were no difference between ERK1/2's mRNA relative expression among low concentration group ,control group and taurine control group,the ERK1/2's mRNA relative expression of low,medium,high concentration manganese groups were lower than those corresponding to the same concentration of manganese in the intervention groups.Conclusions: Manganese can cause oxidative stress injury of hippocampal neurons. Taurine can inhibit the hippocampal neurons oxidative stress caused by manganese, ERK1 / 2 plays an important role in the taurine antagonist hippocampal neurons oxidative stress injury induced by manganese.