In Vivo And Real Time Investigation Of Pharmacokinetics Of Pharmaceuticals By Capillary Electrophoresis

The dissertation consists of two parts. Part 1 describes in vivo and real time investigation of pharmacokinetics of pharmaceuticals in animals by capillary electrophoresis. In part 2, a novel chemiluminescence analytical system was proposed and the feasibility of coupling it to on-line microdialysis sampling for the study of drug-protein interaction.The main contents and some conclusions of the thesis are as follows:Part 1 is divided into three chapters. Chapter 1 is a review dealing with the application of capillary electrophoresis different separation models in pharmacokinetic analysis. Emphasis is placed on the development in the rencent ten years since 1995 especially since 2000.Chapter 2 is composed of 3 sections. The section 1 presents the fundamental theory of pharmacokinetics and the principle of microdialysis technique briefly;The section 2 describes an microdialysis system coupled with capillary electrophoresis for monitoring of ranitidine hydrochloride in rabbit blood. Firstly capillary electrophoresis was developed to determine ranitidine hydrochloride and famotidine. The influences of pH, concentration of buffer, applied voltage, injection time on determination of ranitidine hydrochloride and famotidine was investigated. Under the conditions of phosphate buffer, pH 6.2, applied voltage 10kV, injection pressure 2758Pa, time 4s, it was found that ranitidine hydrochloride and famotidine can be baseline separated. The peak area ratio of ranitidine hydrochloride and famotidine vs concentration of ranitidine hydrochloride was linear over the range of 1.5×10^-7 g·mL^-18.0×10^-6 g·mL^-1 and the limitation of detection was 9.0×10^-8 g·mL^-1 The pharmacokinetic parameters of ranitidine hydrochloride were calculated utilizing...