Pathological Study Of Xin Jiang Hemorrhagic Fever Virus Infection In Suckling Mice

Xin jiang hemorrhagic fever(XHF) is a zoonosis which is caused by Xin jiang hemorrhagic fever virus and transmitted by Hyalomma ticks. Light microscopy, electron microscopy, immunohistochemical staining, in situ hybridization, and RT-PCR were performed to study the pathological changes of suckling mice inoculated with XHF virus by subcutaneous or intraperitoneal injection at different time point. The purpose of this study is to establish a pathological way for diagnosis and study the pathogenesis of Xin jiang hemorrhagic fever primarily.Results: 1. Degeneration and necrosis of hepatocytes were found in bothgroups of animals. There is not obvious inflammation accompanied with the infection and injures of the hepatocytes. Cerebral haemorrhage also can be found in some animals. 2. Virus can be detected by the immunohistochemistry, in situ hybridization and transmission electron microscopy in the cytoplasm of hepatocytes and be detected in hearts, livers, spleens, kidneys and brains by RT-PCR. 3. The injures aggravated gradually from 4 to 7 days after inoculation and the pathological changes at 6 and 7 days are typical. The degeneration and necrosis are always present in the cytoplasm of hepatocytes accompanied with the positive signals of antigen or nuclear acid of XHF virus.Conclusions: 1. Suckling mice are sensitive to XHF virus. Inoculation bysubcutaneous or intraperitoneal injection can both cause injuries. 2. Livers are the main targets of infection. The degeneration and necrosis of hepatocytes are the main pathological changes. 3. The virus can be detected by the combination of immunohistochemistry, in situ hybridization , transmission electron microscopy and RT-PCR. 4.The histopathological change, development, evolution and the distribution of the virus in the animals inoculated by the two different approaches are almost the...