Experimental Study Of Mice On Human Cytomegalovirus Primary Infection In Pregnant Women And Fetuses

Objective To observe the effect of maternal HCMV primary infection on embryo and fetus. The study aimed at providing a scientific method to evaluate the risk of abnormal pregnancy when maternal HCMV primary infection. Methods Firstly we established a model of maternal HCMV primary infection in mice. IgM and IgG of HCMV are negative in mice sera before experiment. HCMV (10~6TCID50 in 0.5ml/mice) was separately injected into the intraperitoneum of 10-week-old female mice on pre-mating, 3, 10, 15 gestation days in experimental group. The equal quantity supernate of HF cell were inoculated in mice of control group at the same times. (1) Observe the mice behavior change postinoculation. (2) Brain, heart, lung, liver, kidney, placenta of fetuses were collected by laparotomy before delivery, then observing pathology through light and electron microscope. (3) HCMV IgM, IgG and DNA were tested by ELISA and PCR in maternal and fetal sera and amniotic fluid. HCMV DNA in meternal sera and amniotic fluid were tested by FQ-PCR. (4) Observe the outcome of pregnancy, level of growth and intelligence development, postnatal mortality and complication. Results (1) Intrauterine infection can happen in three trimesters and pre-mating of maternal HCMV primary infection, and lead to growth retardation, fetal death, abnormity and blastocolysis of embry. The infection rates in group A1, A2, A3, A4 were 31%, 63.5%, 28.5%, 12.5% respectively( P<0.01). The abnormality rates of fetus were 11.1%, 23.4%, 11.3%, 6.8% respectively(P<0.01). (2)The relation of quantity of HCMV DNA in maternal sera and the risk of fetus infection was positive. The risk of congenital HCMV infection increase 11.92 time along with the load of virus in serum from 10~6copies/ml to 10~8copies/ml. (3)The...