The Changes Of Serum Osteocalcin And Leptin Before And After R-Hgh Treatment In Children With Short Stature And The Correlation Between Osteocalcin And Leptin

OBJECTIVE: To study the changes of serum osteocalcin(Ost) andleptin(LEP)before and after rhGH treatment in children with short stature and the correlation between Ost and LEP.METHODS: Serum Ost and LEP were measured in 20 GHD children and18 ISS children before and after 3 months of rhGH therapy,and compared to 20 normal children.The serum levels of Ost and LEP were messured by specific radio-immunoassay(RIA).ALL calculation were performed with SPSSlO.O.Statistical analysis for comparison of data between groups was performed by the Student-Newman-Keuls.The paired-Samples T test was used to compare data before and after treatment. Correlation analysis were performed using pearson correlation coefficient.RESULTS: l.The serum Ost levels was lower in pre-treatment GHDgroup(14.065±4.99ng/ml) than those in control group(31.623±9.67ng/ml ).There was significant difference between the pre-treatment GHD group and the control group (P<0.01) .2. The serum Ost level was lower in the pre-treatment ISS group(13.159±4.026ng/ml) than in control group(31.623 ± 9.67ng/ml ).There was significant difference between the pre-treatment GHD group and the control group (P < 0.01) .3. There was non-significant difference between the pre-treatment GHD group and the pre-treatment ISS group for the serum Ost level (P >0.05 ) .4. The serum Ost level was higher in the pro-treatment GHD group(27.84± 12.39ng/ml) than those in the pre-treatment GHD group .There was significant difference between the two groups (P < 0.01) ;But, the serum Ost level was also lower in the pro-treatment GHD group than in control group.There was significant difference between the pro-treatment GHD group and the control group ((P >0.05) .5. The serum Ost level was higher in the pro-treatment ISS group(21.34± 6.84ng/ml ) than those in the pre-treatment ISS group .There was significant difference between the two groups (P < 0.01) ;But, the serum Ost level was also lower in the pro-treatment ISS group than in control group.There was significant difference between the pro-treatment GHD group and the control group (P < 0.01)6. The serum Ost levels was higher in the pro-treatment GHD group( ) than those in the pro-treatment ISS group .There was significant difference between the two groups (P<0.05)7. The serum LEP levels was higher in pre-treatment GHD group(12.57± 6.91ng/ml ) than those in control group(2.22±0.82ng/ml).There was significant difference between the pre-treatment GHD group and the control group (P < 0.01) .8. The serum LEP level was higher in the pre-treatment ISS group(11.83± 5.90ng/ml) than in control group(2.22±0.82ng/ml).There was significant difference between the pre-treatment GHD group and the control group (P<0.01) .9.There was non-significant difference between the pre-treatment GHD group and the pre-treatment ISS group for the serum LEP level (P >0.05) . 10. The serum LEP level was lower in the pro-treatment GHD group(6.47±3.60ng/ml) than those in the pre-treatment ISS group .There was significant difference between the two groups (P < 0.01) ;But, the serum LEP level was also higher in the pro-treatment GHD group than in control group.There was significant differencebetween the pro-treatment GHD group and the control group (P<0.01) .11. The serum LEP levels was lower in the pro-treatment ISS group(6.47± 3.60ng/ml) than those in the pre-treatment ISS group .There was significant difference between the two groups (P < 0.01) ;But, the serum LEP levels was also higher in the pro-treatment ISS group than in control group.There was significant difference between the pro-treatment ISS group and the control group (P<0.01) .12. The serum LEP levels was lower in the pro-treatment GHD group(6.07± 2.43ng/ml) than those in the pre-treatment ISS group .There was significant difference between the two groups (P >0.05) .13.There was non-significant correlation between the serum levels of Ost and LEP in the pre-treatment GHD group(r=-0.280 ),so in the pre-treatment ISS group(r=-0.181).14. There was non-significant correlation between the serum levels of Ost and LEP in the pro-treatment GHD group(r= r=-0.286 ),so in the pro-treatment ISS group(r=-0.091).15. There was non-significant correlation between the serum levels of Ost and LEP in control group. ( r=-0.185)CONCLUSION 1. The serum Ost levels in the pre-treatment GHD groupand pre-treatment ISS group were both lower than those in control group,which show there have been abnormal bone metabolism of prepubertal children with short stature.2. The serum Ost levels in the pro-treatment GHD group and pro-treatment ISS group were both higher than those in the pre-treatment GHD group and pre-treatment ISS group, which show that rhGH could increased linear growth by increasing the metabolism of bone.But,after 3-month-treatment of rhGH, The serum Ost levels were still lower than those of control group.That indicated the treatment of short stature was a long-term case.3. The serum Ost levels was higher in the pro-treatment GHD group than those in the pro-treatment ISS group ,which showed that the effect of increasing lineargrowth was better in GHD group than in ISS group.4. LEP and GH were both related to eath other,indicated that high LEP level might be one of the reason that developing short stature.5. There was non-significant correlation between high LEP level and abnormal bone metabolism in children with short stature, indicated that serum Ostmight not direct adjust bone cells.