Diagnostic Significance Of Chromoendoscopy And Expression Of P53,P63,P73 Protein In Early Gastric Cancer

Objective 1.To study the diagnostic significance of chromoendoscopy to early gastric cancer. 2. To research expression of P53, P63, P73 protein in the different diseases of gastric mucosa. To investigate the relation between expression of P53,P63,P73 protein and the malignant transformation of gastric mucosa epithelium and their correlation in gastric carcinoma and the relation to histological grade, lymph node metastases, depth of invasion of gastric mucosa, TNM stage in gastric carcinoma.Methods 1 .Thirty-four patients were examined with chromoendoscopy and pathologic.2.Immunohistochemical method was used to detect the expression of P53, P63, P73 protein in 42 cases of gastric, carcinoma, 22 cases of chronic surperficial gastritis, 26 cases of chronic atrophic gastritis, 19 cases of gastric ulcer and 20 cases of atypical hyperplasia and a statistical analysis was made according to clinicopathological data.Results 1 .The positive rate of intestinal metaplasia and dysplasia in staining region was 67.86%. 2.The positive rate of expression of P53 protein in gastric carcinoma was markedly higher than that in chronic surperficial gastritis, that in chronic atrophic gastritis, that in gastric ulcer and that in atypical hyperplasia. The expression rate of p53 protein was 54.76% in gastric carcinoma. Compared with that in chronic surperficial gastritis group, that in chronic atrophic gastritis group, that in gastric ulcer group and atypical hyperplasia group, the difference was significant (P<0.05).The expression rate of p53 protein positively related with differentiation of gastric cancer, depth of invasion ofgastric mucosa, lymph node metastasis and TNM stage (P<0.05).3 The expression rate of p63 protein in gastric carcinoma was 35.71%,which had statistic different compared with those of chronic atrophic gastritis and atypical hyperplasia. The expression rate of p63 protein positively related with differentiation of gastric cancer. But there was no relativity of p63 protein expression with depth of invasion of gastric mucosa, lymph node metastasis and TNM stage of gastric cancer(P>0.05).4. The expression rate of p73 protein in gastric carcinoma was 40.48%, which had statistic different compared with those of chronic surperficial gastritis, chronic atrophic gastritis, gastric ulcer and atypical hyperplasia(P<0.05). The expression rate of p73 protein positively related with differentiation of gastric cancer(P<0.05). But there was no relativity of p73 protein expression with depth of invasion of gastric mucosa, lymph node metastasis and TNM stage of gastric cancer(P>0.05). 5.The positive correlation was present between P53 and P63, P73 and P53 protein expression.Conclusion 1. Staining endoscopy examination can improve the diagnoses of early gastric cancer. 2. P53 protein express highly in gastric cancer. p53 gene mutations play an important role in gastric cancer genesis. It is very useful to investigate P53 protein expression in gastric cancer early diagnosis and its prognosis. 3.P63 and P73 protein are expressed partly in gastric cancer, this suggests that P63 and P73 protein still have relativity with gastric cancer development. Perhaps p63 and p73 gene have the other pattern to take part in the development of gastric cancer, which is different with the traditional suppression gene pattern.4.Abnormal expression of P63 and P73 may be due to mutation of P53.5.Cooperative expression of P53, P63 and P73 protein could be an important index to judge the malignant transformation and clinicopathological process and prognosis.