| Objective Stanniocalcin (STC) is a kind of glycoprotein hormone,which was found primarily in teleostean and lately in hunman beings and mammals.It can adjust the balance of calcium and phosphate by kidney and intestinal system.It also plays an important role in promoting the ending differentiation of the brain neuron,avoiding the damage of brain neuron which was caused by the local shortage of blood and the growth of bones and muscles.The lately research indicates that, STC was of virtural importance in the process of the occur and growth of the human tumor. The imperceptible analysis of the cDNA shows that STC1 up shifts in the primary liver cancer, whereas it down shifts in ovary cancer and galactophore cancer.However, STCl and STC2 are both expressed in the ER positive galactophore cancer. The expression of STC2 was abducted by estrogen. The tumor-restrain-gene BRCA1 can make STC1 up shift.This article mainly discuss on the relationship between the expression of target gene human stanniocalcin-I(hSTC-I) and the stomachcancer and liver cancer in different phases.It aims at discussing the relativity between them and the relationship among the clinical type, stages of pathology and transfer. We can learn about the expression of STCl in different tumor sufferers in order to find out the basis according to the degree of malignant tumor and the prognosis.lt also discuss that whether STCl could be used on the clinical sufferers as a new tumor marker,improve the diagnosis rate of the digestive system cancer and provide the experimental basis of the forepart diagnosis, forepart therapy and the judgement of prognosis.Methods: Collect the vein blood and separate single nucleus cell. Then distill the total RNA of the single nucleus cell with the RNA-distill kits of the QIAGEN Co. Use Oligo-6 as the primer, AMV, reverse-transcripte at 42 ℃ for 45 minutes. Destroy the AMV at 70 ℃, then use STC1,STC2,β-actinl and β-actin2 as expand primer, expand the products of the reverse transcription 30 cycles by PCR. The condition is, pre-denaturalization at 94 ℃ for 180 seconds, denaturalization at 94 ℃ for 40 seconds, reversion at 55 ℃ for 40 seconds, extend at 72℃ for 50 seconds. Electrophorese the products of expandition, then take pictures of it with the Gene genius system. Determine the absorbency of the fluorescence belt by Gene Tools From SynGene software. After that, calculate the relative expression quantity of each STC1 ,with the basis of β-actin lightness of each sample. Make use of SPSS Stat, software to dealwith and analyze the expression of tumor STCl gene and the relativity between the clinical pathology data.Results: The results of the RNA-distill is pure and complete. Only one sample out of the 18 healthy adults and 20 alimentary tract inflammation sufferers is positive. The relative expression quantity of Hstc-1 mRNA of them is 0.065,0.056 by average. 33 liver cancer sufferers' relative expression quantity of hSTC-1 mRNA is 0.736,and the positive rate is 94.0%(31/33), including 23 transferred liver cancer, the positive rate is 100%. 30 gastric cancer sufferers' relative expression quantity of hSTC-1 mRNA is 0.535, the positive rate is 83.3%(25/30), among them, the high-differentiation group's relative expression quantity is 0.428, the positive rate is 83.3%(7/11);the low differentiation group is 0.694 and 94.7%(18/19). With the same 33 liver cancer sufferers, the transferred group's relative expression quantity is 0.805, the positive rate is 100%(23/23); while the non-transferred group's is 0.685 and 80%(8/10). Among the cancer sufferers, healthy adults and the alimentary tract inflammation sufferers, the relative expression quantity is different by static. The same with the high-differentiation group and the low-differentiation group ,the transferred and the non-transferred. Conclusions:1. The hSTC gene is merely express in healthy adults and the alimentary tract inflammation sufferers. In contrary, theexpression level is obviously higher in gastric cancer and liver cancer sufferers.2. The expression level of the hSTC-1 gene in gastric cancer sufferers is relate to the differentiation degree of the tumor. The lower of the differentiation degree, the higher of the expression level.3. The expression level of the hSTC-1 gene in liver cancer sufferers is relate to the transfer or not of the tumor. The expression level of the transferred sufferers is higher than the non-transferred.4. The expression level of the hSTC-1 gene might be seen as thetumor marker of the gastric cancer and the liver cancer. And it is closely relate to the differentiation degree and the transfer of the tumor. |
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