Expression Of Eotaxin And ECP In Mucosa Of Perative Cavity After Nasal Endoscopic Surgery

The recovery in the cavity after Functional endoscopic sinus surgery (FESS) is rather long, else, the recurrence of chronic sinusitis and nasal polyps in some patients puzzled the surgeons. Infiltration of intramucous inflmmatory cells, edema and polypoid hypertension severely hold back the epithelization and prevent the effect, regretly, it's remain unknown. Obvious pathological characters of in nasal polyps and mucosa lie in the broken of of epithelium,the specific gathering and increased activity of Eos. Eotaxin has specific chemotaxis and activity of of eosinophil (Eos). Eosinophil cationic protein (ECP) can injury the nasal epithelium, the contain of ECP may show the activity of Eos. Western-blot, immunohistochemical(IHC) technique and emzyme immunofluorescence(EIF) had been used to detect eotaxin and ECP during the recovery of FESS dynamically, and to analyze the significance of Eos gathering and activation. This study may afford new molecular opinions for the mechanisms of mucous recovery of FESS, consequently help to improve the clinic managements for the cavity.Result:1. Mass observation: 1-2 weeks after FESS:much secrection, hard coagulation, fibrous secrection etc. 4-6 weeks: hypertensions: edema,vesicles,granulations. 10-12 weeks.rare secrection, epithelization.2. IHC and Western-blot dectections of eotaxin: Predominant intracytal expressions in hypertensive vessels and endoepithelium and Eos was noticed, partly of eotaxin outbroke. Weak expressions in normal group, 1-2 w group, 10-12 w group, on the contrary, strong expressed in 4-6 w group and the sinusitis and had certain rules: (1)which in l-2w group obviously descended compared to those before FESS; (2)it reached the peak as preoperative group in 4-6 w ;(3) another descending occurred within 10-12 w, it fell to the level in 1-2 w.3. EIF detection for ECP: ECP in nasal mucosa in 4-6w group obviously stronger than that in 1-2 w group, 10-12 w group and control group, it was similar to the the ECP level in sinusitis.4. Correlation of eotaxin and ECP during the mucous recovery in operative cavity: it positively correlated(0.719, P<0.01).Conclusion:1. Dynamic expressions of eotaxin and ECP in the operative cavities are correspondence to the clinical mucous recovery, it demonstrates the gathering and acting level of Eos closely related to the edema and polyoid hypertension. Eotaxin and ECP may play important role in the recovery of FESS.2. Strong expression of eotaxin and ECP may be involved in the occurrence of chronic sinusitis and nasal polyps.3. The attack to membrane of ECP breakdown the nasal epithelium of operative cavity. Repairing growth and destroy of epithelial cells occurred at the same time after FESS, it probably hold back the epitheliazation.4. Stronger expressions of eotaxin and ECP in 4-6 w group compared to other groups may reveal a critical stage during the epitheliazation and help the clinical attention.