| ObjectiveAt present, the treatment of cerebrovascular diseases, paticular ischemic, has not been an efficacious remedy. Thrombolysis therapy in super early period can recover cerebral blood stream earlier, ameliorate the blood supply of ischemic penumbra, decrease infarct area and save the brain tissue without necrosis and function,but injuries are inevitable with revascu — larization. That is reper-fusion damage The study aims at observating the effects of ultra short wave therapy on brain edema in the super early period, infarct volume, antiapoptosis cell after the middle cerebral artery occlusion and reperfusion and providing theory evidence clinically by utilizing modified Zea - Longa' s method to duplicate the rat model.Materials and MethodsFocal cerebral ischemia in rats was produced by 2h occlusion of the middle cerebral artery (MCAO) and 24h reperfusion. Neurological deficits were evaluated by 0 ~ 4 scales (To select rats). The cerebral water content was determined by wet/dry weighing method. The infarct volume was measured by 2,3 ,5 - triph-enyltetrazolium chloride(TTC) staining technique. The activity of Bcell lympho-ma/Leukemia - 2 ( Bcl - 2 ) in ischemic tissue of brain and morphology changing were analyzed.ResultsThe increase in pathologic injuries as well as the decrease in the activity of Bel - 2 were significant by ultra short wave therapy on rats when the MC AO was performed for 2h and reperfused for3h. . The variety of infarct volume and water con tent is not significant.DiscussionNow it is considered that a few pathopoiesia mechanisms result in cerebral ischemia reperfusion injuries. Among those,free radicals are the most important. Free radicals increasing rapidly can react with un - saturated fatty acid on cell membrane after focal ischemia reperfusion, which form superoxide lipid, damage membrane, lead to cell breaking, destroy blood brain barrier and form cerebral e-dema. Bel - 2 is a kind of antiapoptosis cell whose content change reflects the level of tissue damage. The study indicates brain water content increased when the MCAO was performed for 2h and reperfused for24h, which means free radicals injuries. Compare to control group, ischemic brain Bel -2 decrease (P <0. 05 ) by ultra short wave therapy in super early periodUltra short wave has heat and nonheat effect on human. It hasn' t been known the mechanism of nonheat effect. It has been reported that ultra short wave therapy can abatement the water content of trauma cerebral edema experimentally and protect the limb ischemia reperfusion injuries by decreasing free radicals content and increasing SOD content. The study adopts ultra short wave of nonheat dose to treat ischemicratshead in the super early period of reperfusion We found that brain Bel -2 decreased by ultra short wave therapy on rats when the MCAO was performed for 2h and reperfused for 3h. which is different from other reports that adopts ultra short wave after 6h. We can analogy this difference . Little dose of ultra short wave has a few kinds of therapeutic mechanism on human. It is also a heated question that ultra short wave may excite Vagus nerve to make blood vessel expand and blood stream speed up. The study on the timeof brain blood stream after MCAO indicates;ischemic brain blood stream increases suddenly, then forms " so call n high irrigating situation after reperfusion for 6h,blood steam decreases and forms low irrigating situation;brain blood stream will stabilize 72h later. So it may have protective effects by relieving low irrigation situation, with ultra short wave therapy on rats after reperfusion for 6h. But in the initial stage (3h) when given ultro short wave therapy,the blood steam will increase greatly,so can result in serious damage.In the study, the effect of infarct volume and water content by ultra short wave therapy isn' t apparent. It may be related to less case numbers or larger variation of infarct volume.The method with ultra short wave therapy is simple, economical and safe, so it has a good application perspective. It also needs more study about therapeutic dose,best timing and mechanism.ConclusionUitra short wave has aneuroprotective effect against focal cerebral ischemia repefusion injuries but using it in super early period may be result in serious damage. |
PDF Full Text Request