Expression And Significance Of HIF1α,GLUT1,ER In Endometrial Adenocarcinoma

Objective: To investigate the expression of hypoxiainducible factor 1a (HIF1α ), glucose transporter 1(GLUT1), estrogen receptors (ER) and the possible relationship between HIF1 α ,GLUT1, ER and clinicpathological parameters in endometrial adenocarcinoma. In addition, to study the relationship between hypoxia and ER in endometrial adenocarcinoma.Method: Immunohistochemical staining S-P method was used to detect the expression of HIF1 α , GLUT1, ER in 15 cases of normal endometrium, 17 cases of atypical endometrium and 45 cases of edometrial adenocarcinoma, and analyze the relationship to histologial grade, surgical stage, muscular invasion, lymph node metastasis.Result: The positive expression of HIF1 α , GLUT1 and the negative expression of ER in endometrial adenocarcinoma, atypical endometrium were higher than that in normal endometrium(P<0.05). In endometrial adenocarcinoma tissues, the expression of HIF1 α was significantly correlated with the depth of myometrial invasion and lymphatic metastasis. But there was no association noted between HIF1a expression and histological grade or surgical stage;With the advance of histological grade,surgical stage, muscular invasion and lymphatic metastasis, the positive expression of GLUT1 and the negative expression of ER were increased. However, all of those factors were not correlated with different ages, menopause status. Positive correlation was also found between GLUT1 and HIF1 a expression (r=0.741,P=0.000), Negative correlation was found between GLUT1 and ER expression(R=-0.280, P>0.05), But there was no correlation found between HIF1 a and ER expression(R=-0.455, P< 0.01).Conclusion: 1. The variation of Glutl, HIF1 a and ER expression in endometrial adenocarcinoma might contribute to the occurrence and development of the neoplasms. United detection of HIF1 a , GLUT1 and ER may have effects on early diagnosis, treatment and prognosis of endometrial adenocarcinoma. 2. Reginal hypoxia in endometrial adenocarcinoma is associated with the reduced ER expression, and intermittent hypoxia can cause persistent downregulation. Hypoxia may therefore contribute to the progression of ER negative status and potentially to the development of resistance to endocrine therapy.