The Protective Effect Of Fu Wai Modified Organ Preservation Solution On Endothelium In Isolated Rat Thoracic Aortic Arteries

OBJECTIVE To investigate the effect of Fu Wai Modified (FWM) organ preservation solution on endothelial relaxation and morphology in isolated rat thoracic aortic arteries.METHODS After rat thoracic aortic artery rings were incubated in FWM, HTK , UW and Krebs solution at 4℃ for 4 hours, the structural change of endothelium was evaluated by light and electron microscopy ,Meanwhile pre-contraction induced by prostaglandin F_2a (U46619 30nmol/L) and endothelium-dependent relaxation induced by calcium ionophore (A23187 10^-10—10^-6mol/L)were measured in the presence of indomethacin (7 μmol/L)and LNNA (300 μmol/L) in organ chamber.RESULTS Under light microscopy, there was no significant damage of endothelium among the FWM,HTK,UW group and the control(KH) group; Under electron microscopy ,the damage of endothelium in group FWM and HTK solution was slighter than that in group UW; The maximal relaxation response induced by A23187 in group FWM (72.53% ±10.36%)and group HTK(70.85% ± 10.02%) was higher than that in...