Inhibitory Effect Of The C6 Cells Proliferation By Combining ATRA With IFN-γ

Primary brain tumors (gliomas) are the most common malignant tumors in central nervous system. about 35.26%~60.96% of allkinds of nervous system tumor. They constitute a heterogeneous groupof tumors associated with significant morbidity and mortality,wemajor on the operation to remove the tumor, and assist withradiotherapy, chemotherapy, immunotherapy and gene therapy i.e.Gliomas are characterized by a high invasive potential and display awide diversity of histological features. They derived from glialsupport cells in the brain and the vast majority are thought to be ofastrocytic origin.Even low-grade gliomas infiltrate the entire brain,afeature that precludes their successful therapy. Most glioma will recurafter operation sooner all later, Therefore it is very difficult toeradicate by operation and not sensitive to drugs of accessorytreatment methods to target cells. It has a significance to seek for akind of drugs that has high effective and low side-effect. Chemotherapy is a major strategy of tumor cure, but there are alot of inconveniences for this method. To define the feasibility ofdifferentiation therapy in the treatment of malignant gliomas, thesynergistic apoptosis-inducing effects of atra and IFN-r in gliomacells were studied by flow cytometric analysis. Atra has exhibit a confirmed effect in the clinical anti-tumorarray, especially in Leukemia examination. The IFN-r is a cell factor,which can not directly induce apoptosis of C6 cells;but a significantincrease of the apoptotic rate was observed after ATRA/IFN-rcombination treatment.Our study is major on the research of cooperation between ATRAand IFN-r, which can provide a better way to inhibit glioma cellgrowth and has tremendous application foreground to treat glioma.Objective:To study the inhibiting effect on the growth of rat brain C6glioma and proliferated activity of the rat glioma model beforeand after ATRA and IFN-γtreatment.Methods:C6 glioma cells (1X106/20μl) were seeded with microinfusioninto left armpit of all rats. After raising for 10 days, rat glioma modelswere builted. We classified all these models randomly into 4 groups,each had 8 rats.control group PBS/alcohol (9:1)+Physiological salineexamination group Ⅰ ATRAexamination group Ⅱ IFN-γexamination group Ⅲ ATRA+IFN-γfor each rat. We diluted ATRA into a final concentration of1mg/ml, and IFN-γ1X104IU/ml.In the three treatment groups, they were administrated once a dayfor two weeks at abdominal cavity from the 10th days after cellinoculation. The control group received only 1ml 0.9% NaCl +PBS/alcohol (9:1) every day. The size of glioma were measured, bywhich we make the growth curve .two weeks later after treatment wetake out the tumor and weight, calculate inhibitory rate. Proliferationand morphological change of glioma cells were detected by HEstaining method, flow cytometric analysis.Results:Compared with the control group, the tumor size of rats in thetreatments was decreased. Tumor volumes were measured, In groupⅢ the growth curve was obviously lower than in the other groups.The inhibiting rate of groupⅠ , Ⅱ , Ⅲ was separately 22.2%, 20.9%and 45.9%. The apoptosis ratio of four group was separately 2.66%,8.50%, 7.98% and 19.06% through the analysis of FCM.Conclusions:Both ATRA and IFN-γhave the proliferation inhibitory effect ofC6 glioma measured in volume and weight and promote theapoptosis of tumor cells. While combined with ATRA and IFN-γ canestablish important theoretical basis for its clinical application.