Effects Of ATRA Combined With Nedaplatin On The Proliferation And Apoptosis Of Hepatoma Cell Line Huh-7

Objective:By this experiment, to observed the effects of Alltrans Retinoic Acid (ATRA) and Nedaplatin (NDP) alone and their combined effects for cell proliferation and apoptosis on human hepatoma cell line Huh-7 in vitro, and to investigated the effect combination of chemotherapy medicine to cure hepatoma.Methods:By choosing the medicines at different concentrations of ATRA(10-6,10-5 10-4mol/L) and NDP (1,2,5mg/L)alone and combine effects for hepatoma cell 24,48,72h, Others, the medicines effective final concentration determined by pre-screen test, ATRA(10-5mol/1) combined with NDP (1mg/1) was used to treat hepatoma cells for 24,48,72h. Cell morphology changes was detected using inverted micros-copy; MTT-test was used to estimate the inhibition of proliferation on hepatoma cell line Huh-7;flow cytometry was applied to analyze the effect of cell apoptosis.Results:Experiment with different concentrations of ATRA(10-6,10-5,10-4 mol/1) and NDP (1,2,5mg/1)could markedly inhibit the cell proliferation,and the effect depended on exposure time and concentration, and the effect of combining was additive and synergistic, which had statistical significance comparing with ATRA and Nedaplatin alone(P<0.01).Apoptosis rate by flow cytometry, and the results showed, ATRA effected Huh-7 cells apoptosis rate(48h):control group 0.82±0.15%, ATRA(10-6mol/L) 31.9±1.91%, ATRA (10-5mol/L) 42.57±1.03%, comparing with the experiment group and control group (P<0.05); NDP effected Huh-7 cells apoptosis rate(48h):the control group 0.34±0.07%, the drug groups of NDP(1,2,5 mg/L) were:28.49±0.6%,38.53±0.7% and 46.6±1.1%; and the combined use resulted in synergic effect, NDP (1 mg/1), ATRA (10-5ol/1) and the two combined,they induce the apoptosis rate of Huh-7 at 48h were 28.49%±0.6%,42.57%±1.03%,55.35%±1.30%. So that the combined group comparing with the single groups, P<0.01.Conclusion:Both ATRA and NDP can inhibit cell proliferation and promote cell apoptosis of human hepatoma cell line Huh-7,and comparing with the single groups,ATRA combined with NDP can significantly increase the chemosensitivity on hepatoma cells.